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ORF cDNA Clones/MolecularCloud> | Briefly, to produce antibodies,peptides corresponding to mouse SLCO6C1 (1-14, MAHVRNKKSDDKKA,cysteine added to C-terminus) (GenScript) were synthesized and conjugated to KLH carrier protein. Mouse Slco6c1 ORF clone (#OMu06346) purchased from GenScript was used for generating a plasmid encoding N-terminal Flag- and C-terminal HA-tagged mouse SLCO6C1 by using PCR with the following primers | Get A Quote |
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The sperm calcium channel CatSper plays a central role in successful fertilization as a primary Ca2+ gateway. Here, we applied cryo-electron tomography to visualize the higher-order organization of the native CatSper complex in intact mammalian sperm. The repeating CatSper units form long zigzag-rows along mouse and human sperm flagella. Above each tetrameric channel pore, most of the extracellular domains form a canopy that interconnects to a zigzag-shaped roof. Murine CatSper contains an additional wing-structure connected to the tetrameric channel. The intracellular domains link two neighboring channels to a diagonal array, suggesting a dimer formation. Fitting of an atomic model of isolated monomeric CatSpe... More