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clbP Gene, a Potential New Member of the -Lactamase Family

Int. J. Mol. Sci. 2022-12; 
Adel Azour , Charbel Al-Bayssari , Lucile Pinault , Saïd Azza , Jean-Marc Rolain ,Seydina M. Diene
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Gene Synthesis The ClbP (CAJ76284.1) protein was designed with a Strep-tag at the N-terminus. Synthetic genes optimised for expression in E. coli were purchased from GenScript (Piscataway, NJ, USA) and each construct was cloned into a pET24a (+) plasmid. Get A Quote


The colibactin island (pks) of Escherichia coli formed by 19 genes (55-Kb), encodes nonribosomal peptide (NRP) and polyketide (PK) synthases, which allow the synthesis of colibactin, a suspected hybrid PK-NRP compound that causes damage to DNA in eukaryotic cells. The clbP, an unusual essential gene, is found in the operon structure with the clbS gene in the pks-encoded machinery. Interestingly, the clbP gene has been annotated as a β-lactamase but no previous study has reported its β-lactamase characteristics. In this study, we (i) investigated the β-lactamase properties of the clbP gene in silico by analysing its phylogenetic relationship with bacterial β-lactamase and peptidase enzymes, (ii) compar... More


antibiotics; clbP gene; bacterial toxins