For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

EYFP fusions to HD-Zip IV transcription factors enhance their stability and lead to phenotypic changes in

Plant Signal Behav. 2022-12; 
Bibek Subedi, Kathrin Schrick
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … Taken together, our observations led us to hypothesize that the elevated protein stability of … secondary IgG HRP antibodies (1:3300; GenScript, A00160). Proteins were detected with … Get A Quote


Green fluorescent protein (GFP) and its derivatives are extensively used for labeling cells, monitoring gene expression and/or tracking the localization or interactions of proteins. Previous reports of detrimental effects of fluorescent protein (FP) expression include cytotoxicity and interference with fusion protein function or localization. Only a few studies have documented the fluorescent tag-specific effects in plants. Here, we show that placing an enhanced yellow FP (EYFP) tag on the amino-terminus of GLABRA2 (GL2) and PROTODERMAL FACTOR2 (PDF2), two developmentally important HD-Zip IV transcription factors from , enhances their protein stability. Additionally, expression of EYFP:GL2 not only rescued the ... More


Arabidopsis, EYFP, GLABRA2, HD-Zip IV transcription factors, abaxial curly leaf, protein stability