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Paramecium Polycomb repressive complex 2 physically interacts with the small RNA-binding PIWI protein to repress transposable elements

Dev Cell. 2022-04; 
Caridad Miró-Pina, Olivia Charmant, Takayuki Kawaguchi, Daniel Holoch, Audrey Michaud, Isadora Cohen, Adeline Humbert, Yan Jaszczyszyn, Guillaume Chevreux, Laurence Del Maestro, Slimane Ait-Si-Ali, Olivier Arnaiz, Raphaël Margueron, Sandra Duharcourt
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Proteins, Expression, Isolation and Analysis … All purification steps were monitored either by Coomassie-stained SDS-PAGE or anti-HIS western blot (Genscript #A00186). HMT assays were essentially performed as described in (… Get A Quote


Polycomb repressive complex 2 (PRC2) maintains transcriptionally silent genes in a repressed state via deposition of histone H3K27-trimethyl (me3) marks. PRC2 has also been implicated in silencing transposable elements (TEs), yet how PRC2 is targeted to TEs remains unclear. To address this question, we identified proteins that physically interact with the Paramecium enhancer-of-zeste Ezl1 enzyme, which catalyzes H3K9me3 and H3K27me3 deposition at TEs. We show that the Paramecium PRC2 core complex comprises four subunits, each required in vivo for catalytic activity. We also identify PRC2 cofactors, including the RNA interference (RNAi) effector Ptiwi09, which are necessary to target H3K9me3 and H3K27me3 to TEs... More


Polycomb repressive complex, RNA interference, ciliates, histone modifications, programed DNA elimination, small RNA, transposable elements