For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

Revealing the role of tunable amino acid residues in elastin-like polypeptides (ELPs)-mediated biomimetic silicification

Int J Biol Macromol. 2023-02; 
Yue Qiu , Yuanqing Lin , Bo Zeng , Peiliang Qin , Zhiwei Yi , Guangya Zhang
Products/Services Used Details Operation
Gene Synthesis The genes were synthesized and sequenced by Genscript (Piscataway, NJ) and then cloned into the pET-22b(+) vectors. Get A Quote


Elastin-like polypeptides (ELPs) are attractive materials for the green preparation of silica nanoparticles via biomimetic silicification. However, the critical factors affecting the ELP-mediated silicification remain unclear. Herein, the role of tunable amino acid residues of ELPs in silicification was studied using three ELPs (ELPs[V9F-40], ELPs[KV8F-40], and ELPs[K5V4F-40]) and their fusion proteins (ELPs[V9F-40]-SpyCatcher, ELPs[KV8F-40]-SpyCatcher, and ELPs[K5V4F-40]-SpyCatcher) with different contents of lysine residues. Bioinformatics methods were employed for the first time to reveal the key physicochemical parameters correlated with silicification. The specific activity of ELPs was increased with the p... More


Bioinformatics; Bioinspired synthesis; Biomimetic silicification; Elastin-like polypeptide; Silica nanoparticle; SpyCatcher.