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Single-Molecule Interconversion between Chiral Configurations of Boronate Esters Observed in a Nanoreactor

ACS Nano. 2023-02; 
Xiaoyu Du, Shanyu Zhang, Liying Wang, Yuqin Wang, Pingping Fan, Wendong Jia, Panke Zhang, Shuo Huang
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Isomers of some chemical compounds may be dynamically interconvertible. Due to a lack of sensing methods with a sufficient resolution, however, direct monitoring of such processes can be difficult. Engineered Mycobacterium smegmatis porin A (MspA) nanopores can be applied as nanoreactors so that chemical reactions can be directly monitored. Here, an MspA modified with a phenylboronic acid (PBA) adapter was prepared and was used to observe dynamic interconversion between chiral configurations of boronate esters, which appears as telegraphic switching on top of nanopore events. The mechanism of this behavior was further confirmed by trials with different halogenated catechols, dopamine, adenosine, 1,2-propanediol... More


Mycobacterium smegmatis porin A; boronate ester; chiral configuration; nanopore; single-molecule chemistry.