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A Subunit Vaccine Candidate Composed of Mpox Virus A29L, M1R, A35R, and B6R Elicits Robust Immune Response in Mice

Vaccines.. 2023-11; 
Xuetao Yang , Xidan Yang , Shouwen Du , Congxia Hu , Xiu Yang , Xingyun Wang , Xing Hu , Nino Rcheulishvili , Peng George Wang and Jihui Lin
Products/Services Used Details Operation
Gene Synthesis The coding sequences of MPXV (GenBank No. ON563414) A29L (amino acids 1–110),M1R (amino acids 1–181), B6R (amino acids 20–279), and A35R (amino acids 58–181) were,respectively, added with the His-tag at C terminal, synthesized by GenScript (Nanjing,China), and cloned into pET-9a plasmid between Nde I and BamH I./ In addition, measured with a ToxinSensor Gel Clot Endotoxin Assay Kit (#L00351, GenScript, Nanjing, China), the endotoxin levels of these proteins were less than 0.25 EU per 10 µg, which is far below the permissible limit for immunization. Get A Quote

Abstract

With no specific antiviral drugs and preventive vaccines against Mpox virus (MPXV),the epidemic has led to the declaration of a Public Health Emergency of International Concern.As a developmental direction for new vaccines, studies of subunit vaccines based upon MPXVantigen proteins are lacking. In this study, A29L, M1R, A35R, and B6R of MPXV were expressed and purified from a rokaryotic system. The four MPXV antigen proteins in combination were mixed with aluminum hydroxide or CpG7909 as adjuvant, and subsequently used to inoculate mice. The results of enzyme-linked immunosorbent assay (ELISA), flow cytometry analyses, and enzyme-linked immunospot (ELISPOT) assays indicated that A29L, M1R, A35R, and B6R elici... More

Keywords

Mpox virus; subunit vaccine; CpG7909 adjuvant; prokaryotic expression; immune response