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Prime editor-mediated functional reshaping of ACE2 prevents the entry of multiple human coronaviruses, including SARS-CoV-2 variants

MedComm. . 2023-09; 
Wenwen Zhao, Jifang Li, Xiao Wang, Wei Xu, Bao-Qing Gao, Jiangchao Xiang, Yaofeng Hou, Wei Liu, Jing Wu, Qilian Qi, Jia Wei, Xiaoyu Yang, Lu Lu,Li Yang, Jia Chen, Bei Yang
Products/Services Used Details Operation
Gene Synthesis Primer sets (ACE2_PCR_F/ACE2_PCR_R) were used to amplify the full-length WT human ACE2 (hereafter referred as ACE2) gene from pUC57-Human_ACE2 template (synthesized by GenScript)./The cell lysates were separated by SDS-PAGE (Genscript) and proteins were transferred to nitrocellulose membranes (Thermo Fisher Scientific). Get A Quote
Proteins, Expression, Isolation and Analysis Get A Quote

Abstract

The spike protein of SARS-CoV-2 hijacks the host angiotensin converting enzyme 2 (ACE2) to meditate its entry and is the primary target for vaccine development. Nevertheless, SARS-CoV-2 keeps evolving and the latest Omicron subvariants BQ.1 and XBB have gained exceptional immune evasion potential through mutations in their spike proteins, leading to sharply reduced efficacy of current spike-focused vaccines and therapeutics. Compared with the fastevolving spike protein, targeting host ACE2 offers an alternative antiviral strategy that is more resistant to viral evolution and can even provide broad prevention against SARS-CoV and HCoV-NL63. Here, we use prime editor (PE) to precisely edit ACE2 at structurally s... More

Keywords

broad spectrum, HCoV-NL63, host factor reshaping, prime editing, SARS-CoV, SARS-CoV-2 VOCs