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Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase

Biotechnol Biofuels Bioprod .. 2023-08; 
Danqiong Huang , Chenglong Liu , Mingshan Su , Zhiyong Zeng , Chaogang Wang , Zhangli Hu , Sulin Lou , Hui Li
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Abstract

β-Carotene is one of the economically important carotenoids, having functions as the antioxidant to remove harmful free radicals and as the precursor for vitamin A and other high-valued xanthophyll such as zeaxanthin and astaxanthin. Lycopene cyclase plays an important role in the branching of β-carotene and α-carotene. Aiming to develop the microalgae with enhanced β-carotene productivity, the CrtY gene from bacterium Pantoea agglomerans was integrated into Chlamydomonas reinhardtii. The lycopene-producing E. coli harboring CrtY gene produced 1.59 times of β-carotene than that harboring DsLcyb1 from Dunaliella salina (a microalga with abundant β-carotene), confirming the superior activity of CrtY on β-c... More

Keywords

Cell factory; Chlamydomonas reinhardtii; Eukaryotic microalgae; Lycopene β-cyclase; Prokaryotic gene; β-Carotene.