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The Functional Characteristics and Soluble Expression of Saffron CCD2

Int J Mol Sci. 2023-10; 
Ying Wang, Siqi Li, Ze Zhou, Lifen Sun, Jing Sun, Chuanpu Shen, Ranran Gao, Jingyuan Song, Xiangdong Pu
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Abstract

Crocins are important natural products predominantly obtained from the stigma of saffron, and that can be utilized as a medicinal compound, spice, and colorant with significant promise in the pharmaceutical, food, and cosmetic industries. Carotenoid cleavage dioxygenase 2 (CCD2) is a crucial limiting enzyme that has been reported to be responsible for the cleavage of zeaxanthin in the crocin biosynthetic pathway. However, the catalytic activity of CCD2 on β-carotene/lycopene remains elusive, and the soluble expression of CCD2 remains a big challenge. In this study, we reported the functional characteristics of CCD2, that can catalyze not only zeaxanthin cleavage but also β-carotene and lycopene cleavage. The ... More

Keywords

CsCCD2, crocins, fusion tag, saffron, soluble expression