| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | Primary luminal cells were cultured in advanced DMEM/F12 (Gibco, #12 634 010) supplemented with 5% FBS (Gibco, #10099141), 10 ng mL−1 EGF, 20 ng mL−1 FGF2 (GenScript, #Z03116), 5 μM Y27632 (Sellect, S1049), 4 μg mL−1 Heparin (Thermo, #A16198), 1% sodium pyruvate, 1% L-Ala-Gln, 1% P/S, and 5% Matrigel (Corning,#354234). | Get A Quote |
The genetic basis of vertebrate emergence during metazoan evolution has remained largely unknown. Understanding vertebrate-specific genes, such as the tight junction protein Occludin (Ocln), may help answer this question. Here, it is shown that mammary glands lacking Ocln exhibit retarded epithelial branching, owing to reduced cell proliferation and surface expansion. Interestingly, Ocln regulates mitotic spindle orientation and function, and its loss leads to a range of defects, including prolonged prophase and failed nuclear and/or cytoplasmic division. Mechanistically, Ocln binds to the RabGTPase-11 adaptor FIP5 and recruits recycling endosomes to the centrosome to participate in spindle assembly and functio... More
