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Molecular Biology Reagents> | … Gene sequences for Olfr205 and Olfr740 family members (Olfr740, Olfr741, and Olfr743) were synthesized by Genscript (Nanjing, China) and cloned into the recombinant vector pCI, … | Get A Quote |
Indole is widely present in nature and contributes significantly to the smell of flowers and animal excretion. However, the odor perception mechanism for indole is unclear, despite previous reports suggesting that it activates the Olfr740 family of receptors. In this study, we successfully identified another receptor, Olfr205, that is responsive to indole. Molecular model construction and binding pocket analysis predicted that the A202 residue in transmembrane helix 5 of Olfr205 forms a crucial hydrogen bond with indole, facilitating receptor activation. Additionally, G112 in transmembrane helix 3 of the Olfr740 family is involved in indole activation of receptors. Finally, our mutant function assay showed that... More