For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

Functional characterization of polyphenol oxidase OfPPO2 supports its involvement in parallel biosynthetic pathways of acteoside

Plant J. 2024-06; 
Yating Liu, Xiaoyang Han, Mengya Zhao, Lan Liu, Zixin Deng, Qiu Zhao, Yi Yu
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … The sequence of genes and primers was synthesized by Genscript (Nanjing, China). The protein marker used in the study was TreliefTM Prestained Protein Ladder from Tsingke … Get A Quote

Abstract

Acteoside is a bioactive phenylethanoid glycoside widely distributed throughout the plant kingdom. Because of its two catechol moieties, acteoside displays a variety of beneficial activities. The biosynthetic pathway of acteoside has been largely elucidated, but the assembly logic of two catechol moieties in acteoside remains unclear. Here, we identified a novel polyphenol oxidase OfPPO2 from Osmanthus fragrans, which could hydroxylate various monophenolic substrates, including tyrosine, tyrosol, tyramine, 4-hydroxyphenylacetaldehyde, salidroside, and osmanthuside A, leading to the formation of corresponding catechol-containing intermediates for acteoside biosynthesis. OfPPO2 could also convert osmanthuside B i... More

Keywords

Osmanthus fragrans, acteoside, catechol moieties, parallel routes, polyphenol oxidase