For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

Fit-for-purpose heterodivalent single-domain antibody for gastrointestinal targeting of toxin B from Clostridium difficile

Protein Sci. 2024-07; 
Everardo R Rodriguez Rodriguez, Rune Thorbjørn Nordvang, Marcus Petersson, Jakob Kræmmer Haar Rendsvig, Emma Wenzel Arendrup, Monica L Fernández Quintero, Timothy P Jenkins, Andreas H Laustsen, Sandra Wingaard Thrane
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … added: Protein A-HRP (GenScript, M00089, USA), rabbit anti-chicken IgY-HRP (Invitrogen, 31401, USA), and anti-bovine IgG-HRP (Sigma-Aldrich, A5295, USA) for the detection of the … Get A Quote

Abstract

Single-domain antibodies (sdAbs), such as VHs, are increasingly being developed for gastrointestinal (GI) applications against pathogens to strengthen gut health. However, what constitutes a suitable developability profile for applying these proteins in a gastrointestinal setting remains poorly explored. Here, we describe an in vitro methodology for the identification of sdAb derivatives, more specifically divalent VH constructs, that display extraordinary developability properties for oral delivery and functionality in the GI environment. We showcase this by developing a heterodivalent VH construct that cross-inhibits the toxic activity of the glycosyltransferase domains (GTDs) from three different toxinotype... More

Keywords

Clostridium difficile, antibody, bile salt stability, developability, oral administration, single‐domain antibody