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Proteins, Expression, Isolation and Analysis> | … Protein contents in the samples were quantified and stored at -80 C until use. … protein and 20 µg renal tubule cell lysates were loaded onto Expressplus Page 4% to 12% gel (GenScript). … | Get A Quote |
Plasmodium vivax serological exposure markers (SEMs) have emerged as promising tools for the actionable surveillance and implementation of targeted interventions to accelerate malaria elimination. To determine the dynamic profiles of SEMs in current and past P. vivax infections, we screened and selected 11 P. vivax proteins from 210 putative proteins using protein arrays, with a set of serum samples obtained from patients with acute P. vivax and documented past P. vivax infections. Then we used a murine protein immune model to initially investigate the humoral and memory B cell response involved in the generation of long-lived antibodies. We show that of the 11 proteins, especially C-terminal 42-kDa region of P... More