| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Peptides | … N-terminally biotinylated synthetic FLAG peptide variants were ordered from Genscript with a purity of >=95%. These were dissolved in SPR buffer (150 mM NaCl, 20 mM HEPES pH 7.4… | Get A Quote |
The FLAG-tag/anti-FLAG system is a widely used biochemical tool for protein detection and purification. Anti-FLAG M2 is the most popular antibody against the FLAG-tag, due to its ease of use, versatility, and availability in pure form or as bead conjugate. M2 binds N-terminal, C-terminal and internal FLAG-tags and binding is calcium-independent, but the molecular basis for the FLAG-tag specificity and recognition remains unresolved. Here we present an atomic resolution (1.17 Å) structure of the FLAG peptide in complex with the Fab of anti-FLAG M2, revealing key binding determinants. Five of the eight FLAG peptide residues form direct interactions with paratope residues. The FLAG peptide adopts a 3 helix confo... More
