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CRISPR RNAs/Cas9 Proteins(INACTIVE)> | Two gRNA target sites were tested for each gene. gRNAs were designed using CRISPR-P v2.0 guide RNA tool66 (http://crispr.hzau.edu.cn/CRISPR2/) (Supplementary Table 2), and were synthesized by GenScript (SafeEdit sgRNA). | Get A Quote |
Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs to be restored through a process known as the PSII repair cycle. The detailed molecular mechanism underlying the PSII repair process remains mostly elusive. Here, we report biochemical and structural features of a PSII-repair intermediate complex, likely arrested at an early stage of the PSII repair process in the green alga Chlamydomonas reinhardtii. The complex contains three protein factors associated with a damaged PSII core, namely Thylakoid Enriched Factor 14 (TEF14), Photosystem II Repair Factor 1 (PRF1), and ... More