For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

A novel strategy of co-expressing CXCR5 and IL-7 enhances CAR-T cell effectiveness in osteosarcoma

Frontiers in Immunology. 2024-10; 
Xinhui Hui1†Muhammad Asad Farooq,&#x;Muhammad Asad Farooq1,2†Yiran ChenYiran Chen1Iqra Ajmal,Iqra Ajmal1,2Yaojun Ren,Yaojun Ren1,3Min XueMin Xue1Yuzhou JiYuzhou Ji1Bingtan DuBingtan Du1Shijia WuShijia Wu1Wenzheng Jiang*&#x;Wenzheng Jiang1*‡
Products/Services Used Details Operation
Nucleic Acid Purification & Analysis Primary T cells were positively selected with CD4 and CD8 microbeads, stimulated with Enceed™ (Genscript, Nanjing, China) for 48 hours, and subsequently cultured in X-VIVO 15 Get A Quote

Abstract

Background: Solid tumors are characterized by a low blood supply, complex stromal architecture, and immunosuppressive milieu, which inhibit CAR-T cell entry and survival. CXCR5 has previously been employed to increase CAR-T cell infiltration into CXCL13+ cancers. On the other hand, IL-7 improves the survival and persistence of T cells inside a solid tumor milieu. Methods: We constructed a novel NKG2D-based CAR (C5/IL7-CAR) that co-expressed CXCR5 and IL-7. The human osteosarcoma cell lines U-2 OS, 143B, and Mg63 highly expressed MICA/B and CXCL13, thus presenting a perfect avenue for the present study. Results: Novel CAR-T cells are superior in their activation, degranulation, and cytokine release compete... More

Keywords