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SARS-CoV-2 N protein coordinates viral particle assembly through multiple domains

Journal of Virology. 2024-10; 
Yuewen Han 1, Haiwu Zhou 1, Cong Liu 1, Weiwei Wang 1, Yali Qin 2, Mingzhou Chen
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Plasmids for SARS-CoV-2 Detection and Research The M and N proteins of both SARS-CoV and SARS-CoV-2 were acquired as plasmids from GenScript a Random PS9 was synthesized by GenScript and inserted into pcDNA3.1(+) with mCherry fused at its N-terminus Get A Quote
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Abstract

Increasing evidence suggests that mutations in the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may enhance viral replication by modulating the assembly process. However, the mechanisms governing the selective packaging of viral genomic RNA by the N protein, along with the assembly and budding processes, remain poorly understood. Utilizing a virus-like particles (VLPs) system, we have identified that the C-terminal domain (CTD) of the N protein is essential for its interaction with the membrane (M) protein during budding, crucial for binding and packaging genomic RNA. Notably, the isolated CTD lacks M protein interaction capacity and budding ability. Yet, upon fusion ... More

Keywords

SARS-CoV-2; assembly; genomic RNA; membrane protein; nucleocapsid protein; replication.