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Online detecting living cells released TNF-α and studying intercellular communication using SuperDNA self-assembled conical nanochannel

Chinese Chemical Letters. 2024-10; 
Weiwei Liu , Yu Liu , Zhaoyan Tian , Zhaohan Wang , Hui Liu , Songqin Liu , Yafeng Wu
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Abstract

Nanochannel technology based on ionic current rectification has emerged as a powerful tool for the detection of biomolecules owing to unique advantages. Nevertheless, existing nanochannel sensors mainly focus on the detection of targets in solution or inside the cells, moreover, they only have a single function, greatly limiting their application. Herein, we fabricated SuperDNA self-assembled conical nanochannel, which was clamped in the middle of self-made device for two functions: online detecting living cells released TNF-α and studying intercellular communication. Polyethylene terephthalate (PET) membrane incubated tumor associated macrophages and tumor cells was rolled up and inserted into t... More

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