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Controlled TPCA-1 delivery engineers a pro-tenogenic niche to initiate tendon regeneration by targeting IKKβ/NF-κB signaling

Bioact Mater. 2024-10; 
Jialin Chen , Renwang Sheng , Qingyun Mo , Ludvig J Backman , Zhiyuan Lu , Qiuzi Long , Zhixuan Chen , Zhicheng Cao , Yanan Zhang , Chuanquan Liu , Haotian Zheng , Yu Qi , Mumin Cao , Yunfeng Rui , Wei Zhang
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Recombinant Proteins To evaluate the effect of inflammation on the tenogenic differentia tion of hPDLSCs, rTSPCs, and hBMSCs, cells were cultured in the teno genic medium and treated with IL-1β (0, 0.1, 1, or 10 ng/ml) (GenScript, Z02922), or TNF-α (0, 0.1, 1, or 10 ng/ml) (GenScript, Z02774) for 3 and 7 days. Get A Quote

Abstract

Tendon repair remains challenging due to its poor intrinsic healing capacity, and stem cell therapy has emerged as a promising strategy to promote tendon regeneration. Nevertheless, the inflammatory environment following acute tendon injuries disrupts stem cell differentiation, leading to unsatisfied outcomes. Our study recognized the critical role of NF-κB signaling in activating inflammation and suppressing tenogenic differentiation of stem cells after acute tendon injury via multiomics analysis. TPCA-1, a selective inhibitor of IKKβ/NF-κB signaling, efficiently restored the impaired tenogenesis of stem cells in the inflammatory environment. By developing a microsphere-incorporated hydrogel system for stem... More

Keywords

Acute tendon injury; Multiomics; NF-κB signaling; Stem cell therapy; Tenogenic differentiation.