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Neddylation and Its Target Cullin 3 Are Essential for Adipocyte Differentiation

Cells . 2024-10; 
Hongyi Zhou , Vijay Patel , Robert Rice , Richard Lee , Ha Won Kim , Neal L Weintraub , Huabo Su , Weiqin Chen
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CRISPR Libraries All-in-one pLenti-CRISPR V2 empty vector or gRNAs against murine Nae1, Cul1, Cul2, Cul3, and Cul4a were obtained from GenScript Biotech (Piscataway, NJ, USA). Get A Quote

Abstract

The ongoing obesity epidemic has raised awareness of the complex physiology of adipose tissue. Abnormal adipocyte differentiation results in the development of systemic metabolic disorders such as insulin resistance and diabetes. The conjugation of NEDD8 (neural precursor cell expressed, developmentally downregulated 8) to target protein, termed neddylation, has been shown to mediate adipogenesis. However, much remains unknown about its role in adipogenesis. Here, we demonstrated that neddylation and its targets, the cullin (CUL) family members, are differentially regulated during mouse and human adipogenesis. Inhibition of neddylation by MLN4924 significantly reduced adipogenesis of 3T3-L1 and human stromal va... More

Keywords

adipogenesis; metabolism; neddylation; obesity; post-translational modification