Research Grade AAV Packaging

Beta-testing now open!

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Overview

Adeno-associated virus (AAV) is a nonenveloped, single-stranded DNA virus that can infect both nondividing and dividing cells. AAV is thought to be nonpathogenic to humans and only replicates in the presence of a helper virus. These features have made AAV a useful tool for gene delivery to a wide variety of cell types and an attractive vector for gene therapy.

We are now recruiting beta-testers for our AAV Packaging Services!

If you are using AAV1, AAV2, AAV5, AAV6, AAV8 or AAV-DJ/8 and would like to be part of our beta-testing process, we would love to hear from you!

Our beta-testers are important to us and we are offering heavily discounted prices and additional guarantees. Please reach out to your local sales representative or enter your details along with your desired titer and serotype in the form below and we will get back to you with additional details!

Cell Engineering

Highlight

Multiple Serotype

One Stop Shop

Fast Delivery

Service Specifications

AAV Service	Volume (mL)	Virus Titer	Production Time
AAV1, 2, 5, 6, 8, DJ/8	0.5	>1*10^12 vg/ml	2-3 weeks
AAV1, 2, 5, 6, 8, DJ/8	1	>1*10^12 vg/ml	2-3 weeks
Default QC method	1. qPCR 2. Mycoplasma test

Note:
1. For beta testing phase, we can provide AAV1, 2, 5 , 8, DJ/8 now (Guaranteed titer is >10^12 vg/ml.)
2. AAV9 is exclude from current beta testing program.
3. Currently we will only accept customers who need AAV for in vitro purposes.

Quality Control

Mycoplasma Test

TEM

Endotoxin

qPCR

Sanger Sequencing

SDS-Page

Add-On Services

Add-On Service	Description
Silver Staining	Validate the purity of AAV
Endotoxin	Validate the endotoxin level of AAV and conduct further purify step to reduce animal pyrogen response.
Transmission Electron Microscopy	Validate the empty/full ratio of AAV.
Sanger Sequencing	Validate the integrity of inserted gene of interest (GOI).

Please Fill Out the Form and Become Our Beta Tester

Get in Touch with GenScript Cell Engineering Services

Case Study

Case study 1: AAV purity test

SDS-PAGE analysis of different AAV serotypes with silver staining

(Purified virions were achieved by chromatography)
Case study 2: AAV empty/full ratio detection

Empty / Full Ratio

Titer: 1x10^12-13 gc/ml (qPCR)

Resources

Comparison of different viral vectors

Characteristic	AAV	Retrovirus	Lentivirus
Genome	4.8kb(ssDNA)	9.7kb (ssRNA)	9kb (ssRNA)
Packaging Capacity	4.7kb	3kb	9kb
Infection	Most dividing and non-dividing cells	Only infect mitotically active cells	Most dividing and non-dividing cells
Transduction Efficiency	Moderate	High	Moderate
Integration	Non-integrating	Integrating	Integrating
Expression	Transient or stable	Stable	Stable or Transient
Immunogenicity	Very Low	High	Low
Biosafety	BSL-1	BSL-2	BSL-2
Gene Therapy Strategy	In vivo & In vitro	Ex vivo	In vitro & Ex vivo

AAV Serotype Recommendation

Tissue type	Recommended AAV Serotype
Central Nervous System (CNS)	AAV1, AAV5, AAV8, AAV9, AAV-DJ
Heart	AAV1, AAV8, AAV9
Kidney	AAV2, AAV9
Liver	AAV8, AAV9, AAV-DJ
Lung	AAV5, AAV6, AAV9
Pancreas	AAV8
RPE (Retinal Pigment Epithelium)	AAV1, AAV2, AAV5, AAV8
Skeletal Muscle	AAV1, AAV6, AAV8, AAV9

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.