• Description
  Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency. Note: Concentrated Taq DNA Polymerase (GenScript, E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The dNTP (10 mM) mix may be ordered separately.

• Description
  DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA polymerase is the most common polymerase used for PCR.

• Description
  The Product contain 1000 U Taq DNA Polymerase (Cat. No. E00007) and 0.5 ml of 10 mM dNTP (Cat. No. D0056). The Taq DNA Polymerase is supplied with 10X reaction buffer (Cat. No. B0005) containing 15 mM magnesium chloride.

1 Citations

• Description
  2X Taq Master Mix is a premixed 2X concentrated solution of Taq DNA Polymerase (GenScript, Cat. No. E00007), reaction buffer, MgCl 2 and dNTPs. 2X Taq Master Mix contains all components for PCR, except DNA template and primers. The mixture is optimized for consistent and efficient routine PCR amplifications. It can amplify up to 8 kb fragment from lambda DNA. For a 50 µl reaction, simply add 25 µl of 2X Taq Master Mix to primers, DNA template and PCR-Qualified H2O.

6 Citations

• Description
  Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR.Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see related products).

5 Citations

• Description
  Green Taq DNA Polymerase is designed to increase the stability of the Taq enzyme for more convenient transport and applications. The polymerase (1000 U) is designed for 400 rxns if 2.5 U are used per 50 ul PCR reaction. It can be stable for six months if stored at 4°C or for up to one month without significant loss of activity at ambient temperature. Our technology can increase the stability of Green Taq DNA at higher temperature (72°C). The higher yield PCR product can be harvested in long PCR amplification. Formulation PCR Reaction Buffer (with Mg 2+ ): 100 mM Tris-HCl, 15 mM MgCl 2 , 500 mM KCl and 1% Triton X-100, pH 8.3 (4°C). Enzyme Storage Buffer: 20 mM Tris-HCl, 1 mM DTT,0.1 mM EDTA, 0.1 M KCl, 0.5% Nonidet P40 (v/v), 0.5% Tween 20 (v/v), 50% glycerol(v/v), pH 8.0 (4°C) Applications The applications of Green Taq DNA Polymerase include the following: PCR(For simple templates, this enzyme can be optimized for amplification of PCR products up to 10 kb; However, for complex templates, this enzyme can be used for amplification of PCR products up to 3 kb.) 3'A-tailing of blunt ends (T/A-cloning) Primer extension DNA labeling reactions