GenScript GST tag ELISA Detection Kit is designed to quantitate glutathione-S-transferase (GST) or GST-fusion protein in samples such as cell lysate using a proprietary sandwich ELISA procedure in just 60 minutes. It utilizes two clones of GST monoclonal antibodies that bind to different epitopes of the GST tag protein at the same time. The GST monoclonal antibody, pre-coated on the plate, is used to capture GST tagged protein and the biotin conjugated GST monoclonal antibody (Biotin-GST Mab) is added to interact with GST tagged protein bound on the plate. Streptavidin-HRP is added to interact with Biotin-GST Mab and the final step involves the addition of TMB Substrate that will react with HRP to give product with an absorbance at 450 nm. The quantity of GST tagged protein in sample is determined by comparisons with the absorbance of that of a known GST tagged protein standard curve.

GST Fusion Protein Purification Kit (Cat. No. L00207) is the derivate products of Glutathione Resin which includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.

Glutathione Resin (Cat. No. L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications. Table 1 lists the main characteristics of Glutathione Resin.

The GenScript Glutathione MagBeads are developed for quick and efficient small‐scale purification of recombinant glutathione‐S‐transferase (GST)-tagged fusion proteins from bacteria, yeast and mammalian crude cell lysates.