Spike (S-protein) / Receptor Binding Domain (RBD) mAbs
Our anti-SARS-CoV-2 Spike S1-RBD control mAbs include high-affinity rabbit mAbs, as well as clones that have neutralizing activity and can blog ACE2 binding to RBD.
Additional IgG and IgM mAbs of comparable affinity are available beyond the selection listed here. Extended data (antibody pairing, WB, FACS) is also available. For additional information, contact your account manager, or e-mail [email protected]ipt.com.
| SARS-CoV-2 Spike/RBD mAbs | ||||
|---|---|---|---|---|
| Quantity | Mouse IgG | Mouse IgM | Rabbit IgG | Human Chimeric |
| 0.01 mg* | $99 | $129 | $99 | $99 |
| 0.1 mg | $375 | $429 | $375 | $375 |
| > 0.1 mg | Quote | |||
SARS-CoV-2 S-protein IgG mAbs
| Clone ID | Isotype | Species | EC50 (ng/ml) | ACE2 Blocking IC50 (ng/ml) |
|---|---|---|---|---|
| 6D11F2 | IgG1.K | Mouse | 10.56 | 189.4 |
| 5B7D7 | IgG1.K | Mouse | 12.17 | 163.9 |
| 3B8D4 | IgG2a.K | Mouse | 10.05 | 305.6 |
| 8G3G11 | IgG2b.K | Mouse | 8.44 | 375.2 |
| 9A9C9 | IgG2a.K | Mouse | 8.95 | 275.7 |
| 10G6H5 | IgG1.K | Mouse | 16.75 | 171.1 |
| 11D5D3 | IgG1.K | Mouse | 15.72 | 185.5 |
| 11D5E5 | IgG1.K | Mouse | 15.19 | 227.5 |
| 3B10F3 | IgG1.K | Mouse | 6.35 | 204.3 |
| 4A1D10 | IgG2b.K | Mouse | 5.93 | 365.2 |
| 13E10D5 | IgG1.K | Mouse | 15.44 | |
| BS-M0220 | IgG2a | Mouse | 4.48 | 160.9 |
Please reach out to learn about the human chimeric SARS-CoV-2 Spike S1 IgG antibody we have available.
Epitope Binning
High-affinity mAbs that fall in different epitope bins allow for antibody pairing for ELISA kit development. Request detailed Spike/RBD mAb pairing & clone information here.
| Epitope 1 | 3B8D4 | 5E10G8 | 9A9C9 | 8G3G11 | |||
|---|---|---|---|---|---|---|---|
| Epitope 2 | 5B7D7 | 6D11F2 | 11D5D3 | 11D5E5 | 13E10E12 | 14E7C9 | 14E7C12 |
| Epitope 3 | 3B10F3 | 10A8D2 | 4A1D10 | 5E12F3 | 9B1E8 | 10A8F3 | |
| Epitope 4 | 10G6H5 | ||||||
| Epitope 5 | 3B10B3 | 13E10D5 |
Validation data
Extended data for additional clones, pairing information, and validation assays (WB, FACS) are available on request.
ACE2 blocking assay: Select S-protein IgG mAbs that display ACE2 blocking activity in vitro (IC50 range 160 – 375 ng/ml).
Pseudovirus neutralizing assay: Starred anti-S/RBD mAb clones display the best pseudovirus neutralization activity.
SARS-CoV-2 S-protein IgM mAbs
| Affinity ranking | Clone name | Isotype | Species |
|---|---|---|---|
| 1 | 1D10E2 | IgM | Mouse |
| 2 | 1D10E11 | IgM | Mouse |
| 3 | 12D7C8 | IgM | Mouse |
| 4 | 12D7B7 | IgM | Mouse |
| 5 | 9B12E8 | IgM | Mouse |
| 6 | 11F3B9 | IgM | Mouse |
| 7 | 9B12G9 | IgM | Mouse |
| 8 | 11F3C9 | IgM | Mouse |
| 9 | IgG | IgM | Mouse |
| 10 | 3C4F3 | IgM | Mouse |
Epitope Binning
High-affinity mAbs that fall in different epitope bins allow for antibody pairing for ELISA kit development.
| Epitope 1 | 1D10E2 | 1D10E11 | 9B12G9 | 11F3C9 | 12D7B7 | 12D7C8 |
|---|---|---|---|---|---|---|
| Epitope 2 | 3C4E9 | 3C4F3 | ||||
| Epitope 3 | 9B12E8 |
Validation data
ELISA: N-protein IgM mAbs are high affinity, with EC50 range between 0.7 – 14 ng/ml.
| Clone ID | Isotype | Species | EC50 Value (ng/ml) |
ACE2 Blocking (IC50 (ng/ml)) |
WB | FACS |
|---|---|---|---|---|---|---|
| BS-R2B1 | IgG | Rabbit | 9.57 | +++ | + | |
| BS-R2B2 | IgG | Rabbit | 0.96 | 123.1 | + | +++++ |
| BS-R2B12 | IgG | Rabbit | 1.56 | +++++ | ||
| BS-R2B16 | IgG | Rabbit | 4.28 | + | + | |
| BS-R2B17 | IgG | Rabbit | 1.68 | 171 | + | +++++ |
| BS-R2B27 | IgG | Rabbit | 12.74 | ++ | ||
| BS-R2B30 | IgG | Rabbit | 0.96 | + | +++++ | |
| BS-R2B50 | IgG | Rabbit | 20.12 | +++ | ++ | |
| BS-R1B8 | IgG | Rabbit | 2.670 | +++ | +++++ | |
| 4G6 | IgG | Rabbit | 1.28 | 182.3 | ++ | +++++ |
| 12 D3 | IgG | Rabbit | 0.9 | 119.6 | + | +++++ |
| 39 G6 | IgG | Rabbit | 1.17 | 208.6 | +++++ |
Validation data
ELISA: S-protein rabbit mAbs are high affinity, with many having an EC50 < 3 ng/ml.
SARS-CoV-2 Spike/RBD Human Chimeric mAbs
| Clone ID | Isotype | EC50 for S1-RBD (ng/ml) |
|---|---|---|
| HCBS-R2B2 | IgG | 7.951 |
| HC12D3 | IgG | 6.439 |
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