GenBrick™ Building Blocks for Synthetic Biology
GenScript's GenBrick™ synthesis service provides 100% accurate up to 200 kb long DNA sequences, the fundamental building blocks of synthetic biology. Compared to assembling large DNA constructs from oligos or short DNA segments, which is time-consuming and error-prone, GenScript's GenBrick™ synthesis service delivers full-length synthetic DNA fragments with 100% guaranteed sequence accuracy, free of any mutations or negative clones. Building on our expertise in gene synthesis and molecular biology, GenScript's scientists have developed proprietary technology for accurate and efficient one-step assembly DNA fragments, allowing us to offer the best price and turnaround time available for large synthetic genes: only $0.45/bp and with delivery in as few as 23 business days for custom-synthesized DNA constructs.
Applications of GenBrick™
- Synthetic Genomes: synthetic bacterial and viral genomes are used for a variety of applications, such as gene therapy, vaccine development, and drug delivery.
- Metabolic Engineering: enables efficient production of biomolecules in high quantities with valuable industrial applications.
- Industrial Microbiology: bioengineered microbes for waste remediation and generation of alternative forms of fuel.
- Natural Product Discovery: gene clusters inserted in model organisms to discover novel enzymes for diverse applications.
- Environmental Microbiology/Bioremediation: generation of bioengineered microorganisms to monitor and break down environmental and to develop sustainable agricultural methods.
Why choose GenScript's GenBrick™ Service?
With 17 years of experience in developing advanced technology platforms for gene synthesis, GenScript has an unparalleled record of synthesizing long genes and genes with complex secondary structures. GenBrick™ comes with our 100% sequence verification guarantee, making it a reliable option for synthetic biology research compared to traditional methods. Ultimately, GenBrick™ Gene Synthesis Service makes any synthetic biology research easier, faster, and risk-free.
"GenScript has provided us with a first-rate service. Their customer service has been remarkable. They work on my long gene synthesis projects, and the quality of the gene synthesis is outstanding. Looking forward to working with the team on future projects."
―Simon Anthony, Ph.D.
Assistant Professor, Epidemiology, Center for Infection & Immunity, Columbia University Medical Center
What the Data Say
Objective: To synthesize a gene (Homo sapiens) with 50,245 bp in length, located on chromosome Y.
Strategy: (a) Divided the sequence into 10 x 5 kb fragments containing Ascl restriction sites at 5’ and 3’ ends with 200 bp overlap regions (Fig. 1); (b) all fragments were cloned in pUC57 vector and then transformed in yeast for assembly; (c) after screening (Fig. 2), selected clones were subjected to restriction enzyme digestion and NGS sequencing for final validation.
Results: Results from the gel electrophoresis of digested gene (Fig. 3) and also NGS sequencing (Fig. 4) demonstrated that the 50 kb gene was synthesized without any error and at full length.
Fig. 1 Final construct and assembly strategy.
Fig. 2 Colony screening
Fig. 3 Plasmid gel electrophoresis (lane 1) and digested products by SalI (lane 2).
Fig. 4 NGS sequencing for final validation.
Objective: To synthesize a gene (combination of Bat coronavirus genome and partial Escherichia coli genome) with 100,620 bp in length.
Strategy: (a) Divided the sequence into 4 x 25 kb fragments with each 25 kb fragment first assembled from 5 x 5 kb fragments containing 500 bp overlaps and NotI site (Fig. 1); (b) all fragments were cloned in pUC57 vector followed by transformation in yeast for assembly; (c) after screening (Fig. 2), selected clones were subjected to NGS sequencing for final validation.
Results: Results from the gel electrophoresis of digested gene (Fig. 3) and also NGS sequencing (Fig. 4) demonstrated that the 100 kb gene was synthesized without any error and at full length.
Fig. 1 Final construct and assembly strategy.
Fig. 2 Colony screening.
Fig. 3 Plasmid gel electrophoresis (lane 1) and digested products by AvrII (lane 2).
Fig. 4 NGS sequencing for final validation.
Objective: To synthesize a gene (combination of Bat coronavirus genome, partial Vibrio natriegens genome and partial Vibrio natriegens genome) with 202,155 bp in length.
Strategy: (a) Divided the sequence into 7 x 25 - 35 kb fragments with each fragment first assembled from several 5 kb fragments containing 500 bp overlaps and NotI sites (Fig. 1); (b) all fragments were then cloned in pCC1 or pUC57 vectors followed by transformation in yeast for assembly; (c) after screening (Fig. 2), selected clones were subjected to restriction enzyme digestion and NGS sequencing for final validation.
Results: Results from the gel electrophoresis of digested gene (Fig. 3) and also NGS sequencing (Fig. 4) demonstrated that the 200 kb gene was synthesized without any error and at full length.
Fig. 1
Fig. 2 Colony screening.
Fig. 3 Plasmid was digested by AvrII.
Fig. 4 NGS sequencing for final validation.
GenBrick™ Service Specifications
| Gene Length | Price/bp | Turnaround time | Vector | How to Order |
|---|---|---|---|---|
|
≤8 kb
|
||||
|
8-15 kb
|
$0.45
|
23 business days*
|
pUC57-Brick(+)
See vector map pUC57-Brick
See vector map pUC57-Brick-Kan
See vector map |
|
|
15-20 kb
|
$ 0.55
|
35 business days*
|
||
|
20-30 kb
|
$ 0.65
|
35 business days*
|
||
|
30-50 kb
|
$ 0.75
|
50 business days*
|
||
|
50-100 kb
|
||||
|
100-200 kb
|
||||
|
>200 kb
|
||||
* 23/35/50 business days is typical turnaround time for standard sequences. Complex sequences may take longer; please request a quote for a more accurate estimate for your sequences. Learn more about gene sequence difficulty rules >>
- Downstream services, including cloning, mutagenesis, plasmid prep, and protein expression evaluation are available for an additional cost
- Customers retain all rights to the sequence data and related intellectual property
Delivery Specifications
- 4 μg of lyophilized plasmid containing your gene insert (1 μg for low-copy plasmid) *
- Sequence chromatograms covering your gene (electronic copy)
- Plasmid construct map (electronic copy)
- Quality assurance certificate
Quotation and Ordering
- For quotation requests and questions, you may contact us by email, phone, or via our secured online quotation system.
- Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card. Our gene service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.
- For batch orders, please send us gene sequences in FASTA format or fill out and upload the Quick Quotation Request Form. Our Ph.D level representatives will help manage your projects from sequence design, optimization, synthesis, cloning and beyond without any additional charges (Certain Terms and Conditions Applies).
