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Tagged Protein Purification Resins
Tagged Protein Purification Resins
To simplify the purification of recombinant proteins by affinity chromatography, a tag of protein/peptide or other molecule with known size is usually incorporated into the proteins. The two most commonly used tags are 6×Histidine and Glutathione S-transferase (GST). Biotin and Streptavidin are also frequently attached to proteins of interest to enable simple affinity purification. GenScript provides several affinity resins for convenient and reliable purification of tagged proteins.
| Application | Product | Ligand used | Binding capacity | Size | Cat.No. |
|---|---|---|---|---|---|
| His-tagged protein purification | High Affinity Ni-charged Resin | Ni-NTA-charged resin | 45-60 mg 6xHis-tagged protein (27 kD) /ml settled resin | 10 ml 25 ml 500 ml |
L00223 |
| Ni Resin FF | Ni-IDA-charged FPLC-compatible resin | >40 mg 6xhistidine-tagged protein /ml settled resin | 5 ml 25 ml |
L00465 | |
| High Affinity Ni-Charged Resin FF | Ni-NTA-charged FPLC-compatible resin | >45 mg of histidine-tagged protein /ml settled resin | 5/25/100 ml | L00666 | |
| His-tagged protein purification (Prepacked Column) | High Affinity Ni-Charged Resin FF Prepacked Column | Ni-NTA-charged FPLC-compatible resin in a prepacked column | >45 mg of histidine-tagged protein /ml settled resin | 2 /5 /25 ml | L00683 |
| Ni-IDA Resin FF Prepacked Column | Ni-IDA-charged FPLC-compatible resin in a prepacked column | >40 mg of histidine-tagged protein /ml settled resin | 2 /5 /25 ml | L00684 | |
| GST fusion protein purification | Glutathione Resin | Glutathione | 35-45 mg GST-tagged protein (26 kD) /ml settled resin | 10 ml | L00206 |
| GST Fusion Protein Purification Kit | 1 kit | L00207 | |||
| Purification of DYKDDDDK tagged protein | MonoRab™ Anti-DYKDDDDK Affinity Resin | MonoRab™ Anti-DYKDDDDK antibody | Approximately 1.5 mg DYKDDDDK-tagged protein/ml settled resin | 1 ml 5 ml 25 ml |
L00766 |
| Anti-DYKDDDDK G1 Affinity Resin | Anti-DYKDDDDK G1 antibody | Approximately 1 mg DYKDDDDK-tagged protein/ml settled resin | 1 ml 5 ml 10 ml 25 ml |
L00432 | |
| Purification of biotinylated protein, antibody, lectin etc. | Streptavidin Resin | Streptavidin | 120 nmol D-Binotin/ml settled resin | 5 ml | L00353 |
| Immobilization of sulfhydryl-containing peptides, proteins and other ligands for following purification | High-Affinity Iodoacetyl Resin | >9 mg Goat IgG/ml settled resin | 10 ml | L00403 | |
| 1 kit | L00404 | ||||
| High-Affinity Antibody Purification Kit | |||||
| Immunoprecipitation | Anti-His Affinity Resin | THE™ anti-His monoclonal antibody | >0.8 mg of histidine-tagged protein /ml settled resin | 1/5 ml | L00439 |
| Chicken IgY Precipitating Resin | Goat Anti-Chicken IgY | Immunoprecipitation of chicken IgY or the enrichment of chicken primary IgY‐protein complexes | 1 ml | L00405 | |
| Anti-DYKDDDDK IP Resin | Mouse anti-DYKDDDDK monoclonal antibody | Immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex | 1 ml | L00425 |
Ordering Information
Tagged Protein Purification Resins
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00223-500 |
High Affinity Ni-Charged Resin - 500 ml Resin (Total 1000 ml) |
$2480.00 |
|
L00223-25 |
High Affinity Ni-Charged Resin - 25 ml Resin (Total 50 ml) |
$168.00 |
|
L00223-10 |
High Affinity Ni-Charged Resin - 10 ml Resin (Total 20 ml) |
$80.00 |
|
L00206 |
Glutathione Resin - 10 ml Resin (Total 20 ml) |
$107.80 |
|
L00353 |
Streptavidin Resin - 5 ml Resin (Total 10 ml) |
$152.90 |
|
L00432-5 |
Anti-DYKDDDDK G1 Affinity Resin - 5 ml Resin (Total 10 ml) |
$663.00 |
|
L00432-25 |
Anti-DYKDDDDK G1 Affinity Resin - 25 ml Resin (Total 50 ml) |
$2591.00 |
|
L00432-10 |
Anti-DYKDDDDK G1 Affinity Resin - 10 ml Resin (Total 20 ml) |
$1193.00 |
|
L00432-1 |
Anti-DYKDDDDK G1 Affinity Resin - 1 ml Resin (Total 2 ml) |
$281.00 |
|
L00207 |
GST Fusion Protein Purification Kit - 1 kit |
$139.00 |
|
L00465-5 |
Ni Resin FF - 5 ml Resin (Total 10 ml) |
$49.00 |
|
L00465-25 |
Ni Resin FF - 25 ml Resin (Total 50 ml) |
$209.00 |
|
L00666-5 |
High Affinity Ni-Charged Resin FF - 5 ml Resin (Total 10 ml) |
$62.00 |
|
L00666-25 |
High Affinity Ni-Charged Resin FF - 25 ml Resin (Total 50 ml) |
$253.00 |
|
L00666-100 |
High Affinity Ni-Charged Resin FF - 100 ml Resin (Total 200 ml) |
$849.00 |
|
L00683-55 |
High Affinity Ni-Charged Resin FF Prepacked Column - 5 ml x 5 |
$448.00 |
|
L00683-51 |
High Affinity Ni-Charged Resin FF Prepacked Column - 5 ml x 1 |
$112.00 |
|
L00683-12 |
High Affinity Ni-Charged Resin FF Prepacked Column - 1 ml x 2 |
$65.00 |
|
L00684-55 |
Ni-IDA Resin FF Prepacked Column - 5 ml x 5 |
$425.00 |
|
L00684-51 |
Ni-IDA Resin FF Prepacked Column - 5 ml x 1 |
$95.00 |
|
L00684-12 |
Ni-IDA Resin FF Prepacked Column - 1 ml x 2 |
$45.00 |
Resins for Immunoprecipitation
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
L00439-5 |
Anti-His Affinity Resin - 5 ml Resin (Total 10 ml) |
$1599.00 |
|
L00439-1 |
Anti-His Affinity Resin - 1 ml Resin (Total 2 ml) |
$399.00 |
|
L00425 |
Anti-DYKDDDDK IP Resin - 1 ml Resin (Total 2 ml) |
$199.00 |
Related Products
 | Protein A, G and L Resins: routine purification of antibodies from serum, ascites fluid and cell culture supernatants. |
| High-Affinity Iodoacetyl Resin and High-Affinity Antibody Purification Kit:covalent immobilization of ligands containing sulfhydryl groups for following affinity purification. |
