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Protein Purification Resins

Technical Resources

24 hour

Tagged Protein Purification Resins

To simplify the purification of recombinant proteins by affinity chromatography, a tag of protein/peptide or other molecule with known size is usually incorporated into the proteins. The two most commonly used tags are 6×Histidine and Glutathione S-transferase (GST). Biotin and Streptavidin are also frequently attached to proteins of interest to enable simple affinity purification. GenScript provides several affinity resins for convenient and reliable purification of tagged proteins.

Application Product Ligand used Binding capacity Size Cat.No.
His-tagged protein purification High Affinity Ni-charged Resin Ni-NTA-charged resin 45-60 mg 6xHis-tagged protein (27 kD) /ml settled resin 10 ml
25 ml
500 ml		 L00223
Ni Resin FF Ni-IDA-charged FPLC-compatible resin >40 mg 6xhistidine-tagged protein /ml settled resin 5 ml
25 ml		 L00465
High Affinity Ni-Charged Resin FF Ni-NTA-charged FPLC-compatible resin >45 mg of histidine-tagged protein /ml settled resin 5/25/100 ml L00666
His-tagged protein purification (Prepacked Column) High Affinity Ni-Charged Resin FF Prepacked Column Ni-NTA-charged FPLC-compatible resin in a prepacked column >45 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00683
Ni-IDA Resin FF Prepacked Column Ni-IDA-charged FPLC-compatible resin in a prepacked column >40 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00684
GST fusion protein purification Glutathione Resin Glutathione 35-45 mg GST-tagged protein (26 kD) /ml settled resin  10 ml L00206
GST Fusion Protein Purification Kit 1 kit L00207
Purification of DYKDDDDK tagged protein MonoRab™ Anti-DYKDDDDK Affinity Resin MonoRab™ Anti-DYKDDDDK antibody Approximately 1.5 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
25 ml		 L00766
Anti-DYKDDDDK G1 Affinity Resin Anti-DYKDDDDK G1 antibody Approximately 1 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
10 ml
25 ml		 L00432
Purification of biotinylated protein, antibody, lectin etc. Streptavidin Resin Streptavidin 120 nmol D-Binotin/ml settled resin 5 ml L00353
Immobilization of sulfhydryl-containing peptides, proteins and other ligands for following purification High-Affinity Iodoacetyl Resin 　 >9 mg Goat IgG/ml settled resin 10 ml L00403
1 kit L00404
High-Affinity Antibody Purification Kit
Immunoprecipitation Anti-His Affinity Resin THE™ anti-His monoclonal antibody >0.8 mg of histidine-tagged protein /ml settled resin 1/5 ml L00439
Chicken IgY Precipitating Resin Goat Anti-Chicken IgY Immunoprecipitation of chicken IgY or the enrichment of chicken primary IgY‐protein complexes 1 ml L00405
Anti-DYKDDDDK IP Resin Mouse anti-DYKDDDDK monoclonal antibody Immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex 1 ml L00425

Ordering Information

Tagged Protein Purification Resins

Cat. No. Product Name Quantity Price
L00223-500
 High Affinity Ni-Charged Resin - 500 ml
$2480.00
L00223-25
 High Affinity Ni-Charged Resin - 25 ml
$168.00
L00223-10
 High Affinity Ni-Charged Resin - 10 ml
$80.00
L00206
 Glutathione Resin - 10 ml
$98.00
L00353
 Streptavidin Resin - 5 ml
$139.00
L00432-5
 Anti-DYKDDDDK G1 Affinity Resin - 5 ml
$663.00
L00432-25
 Anti-DYKDDDDK G1 Affinity Resin - 25 ml
$2591.00
L00432-10
 Anti-DYKDDDDK G1 Affinity Resin - 10 ml
$1193.00
L00432-1
 Anti-DYKDDDDK G1 Affinity Resin - 1 ml
$281.00
L00207
 GST Fusion Protein Purification Kit - 1 kit
$139.00
L00465-5
 Ni Resin FF - 5 ml
$49.00
L00465-25
 Ni Resin FF - 25 ml
$209.00
L00666-5
 High Affinity Ni-Charged Resin FF - 5 ml
$62.00
L00666-25
 High Affinity Ni-Charged Resin FF - 25 ml
$253.00
L00666-100
 High Affinity Ni-Charged Resin FF - 100 ml
$849.00
L00683-55
 High Affinity Ni-Charged Resin FF Prepacked Column - 5 ml x 5
$448.00
L00683-51
 High Affinity Ni-Charged Resin FF Prepacked Column - 5 ml x 1
$112.00
L00683-12
 High Affinity Ni-Charged Resin FF Prepacked Column - 1 ml x 2
$65.00
L00684-55
 Ni-IDA Resin FF Prepacked Column - 5 ml x 5
$425.00
L00684-51
 Ni-IDA Resin FF Prepacked Column - 5 ml x 1
$95.00
L00684-12
 Ni-IDA Resin FF Prepacked Column - 1 ml x 2
$45.00
 

Resins for Immunoprecipitation

Cat. No. Product Name Quantity Price
L00439-5
 Anti-His Affinity Resin - 5 ml
$1599.00
L00439-1
 Anti-His Affinity Resin - 1 ml
$399.00
L00425
 Anti-DYKDDDDK IP Resin - 1 ml
$199.00
 

Related Products

Protein A, G and L Resins: routine purification of antibodies from serum, ascites fluid and cell culture supernatants.
High-Affinity Iodoacetyl Resin and High-Affinity Antibody Purification Kit:covalent immobilization of ligands containing sulfhydryl groups for following affinity purification.