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GenScript Online PCR Primers Designs Tool

Basic Standard Advanced

** This online tool designs PCR primers for you.

Primer Name: Number To Return:

Sequence:

Left primer                  Hybridization  probe                  Right primer

Left Primer Sequence Input:
Right Primer Sequence Input:
Targets:
Included Regions:
Excluded Regions:
Start Codon Position:
Sequence Quality:

Min Sequence Quality:      Min End Sequence Quality:

Sequence Quality Range Min:      Sequence Quality Range Max:

Primer Condition Details

  Min Opt Max
Primer Size
Primer Tm
Primer GC%
Max Tm Difference: Max Self Comp:
Max #N's: Max 3's Self Comp:
Max Poly-X: GC Clamp:
Inside Target Penalty: Outside Target Penalty:
First Base Index: Salt Concentration:
Annealing Oligo Concentration: Max 3'Stability:
Max Mispriming: Pair Max Mispriming:

Primer Product Details

MinOptMax
Product Size:
Product Tm:

Objective Function Penalty Weights for Primers

Tm Lt: Gt:
Size Lt: Gt:
GC% Lt: Gt:
Self Complementarity
3' Self Complementarity
#N's
Mispriming
Sequence Quality
End Sequence Quality
Position Penalty
End Stability

Objective Function Penalty Weights for Primer Pairs

Product Size Lt: Gt:
Product Tm Lt: Gt:
Tm Difference
Any Complementarity
3' Complementarity
Pair Mispriming
Primer Penalty Weight
Hyb Oligo Penalty Weight

 
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