Genscript Advanced Primer Design

Basic Standard Advanced

** This online tool designs PCR primers for you.

Primer Name: Number To Return:

Sequence:

Left primer Hybridization probe Right primer

Min Sequence Quality: Min End Sequence Quality:

Sequence Quality Range Min: Sequence Quality Range Max:

Max Tm Difference:		Max Self Comp:
Max #N's:		Max 3's Self Comp:
Max Poly-X:		GC Clamp:
Inside Target Penalty:		Outside Target Penalty:
First Base Index:		Salt Concentration:
Annealing Oligo Concentration:		Max 3'Stability:
Max Mispriming:		Pair Max Mispriming:

	Min	Opt	Max
Product Size:
Product Tm:

Objective Function Penalty Weights for Primers

Objective Function Penalty Weights for Primer Pairs

Product Size	Lt:		Gt:
Product Tm	Lt:		Gt: