GenScript Real-time PCR (TaqMan) Primer Design

** This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments. You can customize the potential PCR amplicon's size range, Tm (melting temperature) for the primers and probes, as well as the organism. You can also decide how many Primer/Probe sets you want the tool to return to you. We recommend you using the GenBank Accession to input your target sequence. However, you can choose to input the sequences manually in raw format.

Number of Primer/Probe Sets to Return (Limit 20 Sets):

PCR Amplicon Size Range:

Primer Tm: Minimum Optimum Maximum °C

Probe Tm: Minimum Optimum Maximum °C


Pick Primer/Probe Crossing Exon Junction

When Pick Primer/Probe Crossing Exon Junction is selected, the exon regions must be defined. If only raw sequence is provided, the sequence will be mapped on the genome (human, mouse or rat at present) sequences to locate the exon boundaries. Besides, there are other two ways to specify exon regions:

  • The best way is to fill in GenBank Accession with the accession number (e.g. NM_145027 , please note the accession number is case-sensitive and should not contain version suffix like '.1' or '.2'). The sequence will be fetched from NCBI data center, and the exon regions are already defined in sequence annotations.
  • Manually specify the exon junctions with ':' in the input sequence (e.g. 'ACGCGCG:CGTACG')

Target Nucleotide Sequences:

GenBank Accession:

or Paste in the DNA Sequence in raw format: