This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments.

  • Use the default settings to get the results in seconds.
  • Customize any criterion to optimize the results.

When Pick Primer/Probe Crossing Exon Junction is selected, the exon regions must be defined. If only raw sequence is provided, the sequence will be mapped on the genome (human, mouse or rat at present) sequences to locate the exon boundaries.

  • Manually specify the exon junctions with ':' in the input sequence (e.g. 'ACGCGCG:CGTACG')

Primer Tm

Minimum °C

Optimum

Maximum °C

Probe Tm

Minimum °C

Optimum

Maximum °C

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