Trimer Oligonucleotides Synthesis

Service

Case Study

• Proportional mutation with high uniform distribution

  Case 1. The target protein has 349 AA and needs saturation mutations at 5 sites, theoretical diversity is 20⁵ = 3.2 X 10⁶

  Results:

  • The sequence coverage is 100%
  • The proportional distribution of 20 amino acids at 5 saturation mutation sites is highly uniform

  

  Case 2. The target protein has 123 AA and needs saturation mutations at 2 sites, theoretical diversity is 20² = 400

  Results:

  • The sequence coverage is 100%
  • The distribution of mutations is highly uniform as 90% / 10% as low as 1.91

  
• Non-proportional mutation matches customized request

  Case 3. The target protein needs a different ratio of mutations at 11 sites

  Results:

  • Amino acid distribution in diverse ratios highly match with design proportions, with low deviation

  

Applications

A DNA library synthesized using trimer oligonucleotides can be utilized to construct mutant libraries for further screening and research as shown below. This may be a more cost-effective and flexible solution.