GenScript TurboCHO™ Platform
An Unparalleled Platform for Bi-specific Antibody Expression

70+

different formats of bsAb produced

5,000+

bsAb projects delivered

6+

optimized processes provided

Bi-specific antibodies (bsAbs) demonstrate novel functionalities that yield remarkable promise in improving the drug therapeutic efficacy through the recognition and targeting of two different antigens or epitopes. Although the optimized purification of mAbs serve as a good starting point for the purification of bsAbs, further optimization cannot be fully eliminated due to the differences in their intrinsic structural.

At GenScript, with the advanced 1) Specialized format design, 2) AI-driven codon optimization, 3) Optimized plasmid ratio, 4) Optimized production process, 5) Proprietary TurboCHO™cell line and 6) Multiple purification columns, GenScript can handle your bsAb projects from micrograms-level to grams-level seamlessly.

The table below is interactive!

The bsAbs in the table are classified by fragment, symmetric and asymmetric, Click on a format to see more details of the molecule: Manufacturability score, recommend purification step, typical yield and SEC-HPLC purity.

Bi-specific Antibody Fragments

1 Diabody

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

2 scDb (sc-Diabody)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

3 TandAb

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

4 VHH-Protein

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

5 Fab-VHH

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

6 Fab-VHHn

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

7 BiTE

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

8 Fab-Protein

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

9 BiTE-Protein

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

10 Multiple VHH

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

11 scFv-Fab

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

12 Fab-scFv

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

13 scFv2-Fab

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

14 Fab-scFv2

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

15 scFv-VHH-scFv

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

16 Fab-Fab

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

17 scFv-Fab-Fab

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

Asymmetric Bi-specific Antibody

1 Heterodimeric IgG

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

2 Heterodimeric IgG(KiH assembly)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

3 Duobody (FAE)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

4 Heterodimeric IgG(Common LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

5 CrossMabCH1-CL

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

6 CrossMabVH-VL

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

7 Fab-IgG (CrossMabVH-VL)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

8 IgG-Fab (CrossMabCH1-CL)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

9 Heterodimeric scFv-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

10 BiTE-Fc (KiH)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

11 scFv/BiTE-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

12 Fab/BiTE-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

13 Fab/scFv-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

14 scFv-Fab/scFv-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

15 Fab-Fc-scFv/scFv-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 3
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

16 Fab-Fc/scFv-Fc-scFv

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

17 Heterodimeric scFab-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

18 Fab/scFab-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

19 DART-Fc (KiH)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

20 Fab/protein-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

21 Heterodimeric IgG-protein

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

22 Heterodimeric IgG-protein complex

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

23 Heterodimeric VHH-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

24 Heterodimeric VHH-IgG (HC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

25 Heterodimeric IgG-VHH (HC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

26 Fab/Diabody-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

27 scFab/BiTE-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

28 Fab-Fc/scFv-VHH-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

29 Fab/VHH-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

30 Fab-Fc-scFv/VHH-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

31 Fab/VHH2-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

Symmetric Bi-specific Antibody

1 IgG-scFv (HC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

2 IgG-scFv (LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

3 scFv-IgG (HC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

4 scFv-IgG (LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

5 Fab-scFv-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

6 IgG-scFv (HC+LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

7 IgG-BiTE (HC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
2
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

8 scFv-IgG (HC+LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

9 scFv-Fc-scFv

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

10 BiTE-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

11 Diabody-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

12 scDb-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

13 DVD-Ig

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

14 VHH-Fc-VHH

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

15 Multiple VHH-Fc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

16 VHH-IgG (HC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

17 VHH-IgG (LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

18 VHH-IgG (HC+LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Moderate

19 IgG-VHH (HC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
4
Recommend purification step(s) 1
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
High

20 IgG-VHH (LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

21 IgG-VHH (HC+LC)

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

22 Protein-IgG

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
Data to be collected
Recommend purification step(s)
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L

23 BiTE-scFc

Manufacturability
Score 1-5 according to bsAb’s expression level and purification difficulty defined by GenScript
(e.g. score 1 represents the most difficult)
3
Recommend purification step(s) 2
Typical yield
Low: <50mg/L
Moderate: 50 – 200mg/L
High: >200mg/L
Low

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