ONE-HOUR Western™ Basic Kits

ONE-HOUR Western™ Basic Kits are the simplified version of ONE-HOUR Western™ Standard Kits. The WestClear™ Nitrocellulose Membrane, ChromoSensor™ One Solution TMB Substrate and LumiSensor™ Chemiluminescent HRP Substrate are not included in the package. It is economical to choose these kits if you have surplus accessories in hand. Associated with GenScript's accessories, ONE-HOUR Western™ Basic Kits can perform as excellent as ONE-HOUR Western™ Standard Kits.

To obtain best results and reduce undesirable variations such as high background, we strongly recommend that the following reagents be used with ONE-HOUR Western™ Basic Kit:

• GenScript WestClear™ Nitrocellulose Membrane (L00224A60).
• GenScript LumiSensor™ Chemiluminescent HRP Substrate Kit (L00221V60).
• GenScript ChromoSensor™ One Solution TMB Substrate (L00222V60).

Key Features

• Easy to perform: Fewer steps mean fewer chances for human error.
• High sensitivity: The sensitivity is comparable to or even better better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
• Reproducible results: The kit produces highly reproducible results.
• Far less optimization than the classical three-step method.
• Secondary antibody is included.

Applications

• Protein/antigen detection
• Evaluation of protein expression
• Antibody/antigen titration
• Analysis and comparison of primary antibody activity levels

Ordering Information