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Optimized and ready-to-go system for Western analysis
With GenScript proprietary Western detection technology, ONE-HOUR Western™ Standard Kits reduce the total analysis time for a Western blot or Dot blot to just one hour. The kits contain all the necessary reagents, buffers, nitrocellulose membranes and HRP substrates for performing Western blot and is compatible with both chemiluminescent substrates and chromogenic substrates, such as DAB and TMB.
Western blot detection of housekeeping protein α-tubulin:
Western blot detection of α-Tubulin using the ONE-HOUR Western™ Standard Kit (Cat. No. L00204C) and α-tubulin polyclonal antibody (Abcam, ab4074). 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and 0.08 μg of Hela cell lysates (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensor™ Chemiluminescent HRP Substrate included in the kit.
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Please contact us (phone: 1-877-436-7274 or email: firstname.lastname@example.org),if you need immediate assistance.