ONE-HOUR Western Detection System - Western blot Kits, Western Detection kits

ONE-HOUR Western™ Detection System

The ONE-HOUR Western™ Detection System is designed to produce high signal with low background for quick and clear western analysis of proteins. GenScript's breakthrough ONE-HOUR Western™ technology simplifies the classical western blot analysis by skipping secondary antibody binding and washing steps. The kits reduce the total western blot analysis time from 4.5 hours down to only one hour.

Easy to perform: Quick and simple procedure.
High sensitivity: The sensitivity of the ONE-HOUR Western™ is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used.
Highly reproducible results.
Less optimization needed than the classical method.
Secondary antibody is included.
No special labeling required for primary antibody.

Examples

A comparison of the ONE-HOUR Western™ Kit to the classical three-step Western procedure is shown below.

After protein transfer from gel to membrane, the users may simply incubate the membrane blot in the ONE-HOUR Pretreatment Solution for five minutes and then in WB Solution with their primary antibody for 40 minutes. We offer a selection of WB Solutions containing secondary antibodies to most commonly used primaries (rabbit, mouse, goat). After three five-minute washes, the membrane is ready to be developed. The entire procedure takes only one hour.

ONE-HOUR Western™ Basic Kits

	Cat. No.	Product Name	Price
	L00204	ONE-HOUR Western^TM Basic Kit (Rabbit) - 1 Kit (5 Assays)	$60.00
	L00205	ONE-HOUR Western^TM Basic Kit (Mouse) - 1 Kit (5 Assays)	$60.00

ONE-HOUR Western™ Standard Kits

Cat. No.	Product Name	Price
L00204C-1	ONE-HOUR Western^TM Standard Kit (Rabbit) - 1 Kit (5 assays)	$75.00
L00205C-1	ONE-HOUR Western^TM Standard Kit (Mouse) - 1 Kit (5 assays)	$75.00
L00204T	ONE-HOUR Western^TM Standard Kit Using TMB (Rabbit) - 1 Kit (5 Assays)	$75.00
L00205T	ONE-HOUR Western^TM Standard Kit Using TMB (Mouse) - 1 Kit (5 Assays)	$75.00

ONE-HOUR Western™ Advanced Kits

	Cat. No.	Product Name	Price
	L00241	ONE-HOUR Western^TM Advanced Kit (Rabbit) - 1 Kit (5 Assays)	$125.00
	L00242	ONE-HOUR Western^TM Advanced Kit (Mouse) - 1 Kit (5 Assays)	$125.00

Western QuickBlock Kit

	Cat. No.	Product Name	Price
	L00276	Western Quick Block Kit - 1 kit	$60.00

LumiSensor™ Chemiluminescent HRP Substrate Kit

Cat. No.	Product Name	Price
L00221V60	LumiSensor^TM HRP Substrate Kit - 1 Kit (2 X 30ml)	$55.00
L00221V300	LumiSensor^TM HRP Substrate Kit - 1 Kit (2 X 150ml)	$145.00
L00221V500	LumiSensor^TM HRP Substrate Kit - 1 Kit (2 X 250ml)	$235.00

ChromoSensor™ One-Solution TMB Substrate

Cat. No.	Product Name	Price
L00222V125	ChromoSensor^TM One-Solution TMB Substrate - 1 pk (125ml)	$39.00
L00222V250	ChromoSensor^TM One-Solution TMB Substrate - 1 pk (250ml)	$68.00
L00222V500	ChromoSensor^TM One-Solution TMB Substrate - 1 pk (500ml)	$119.00

	ONE-HOUR Western™ Basic Kits		ONE-HOUR Western™ Standard Kits				ONE-HOUR Western™ Advanced Kits
	ONE-HOUR Western™ Basic Kits		Chemiluminescent		Colorimetric		ONE-HOUR Western™ Advanced Kits
Components	L00204 Rabbit	L00205 Mouse	L00204C Rabbit	L00205C Mouse	L00204T Rabbit	L00205T Mouse	L00241 Rabbit	L00242 Mouse
Pretreat Solution A (50 ml)	√	√	√	√	√	√	√	√
Pretreat Solution B (50 ml)	√	√	√	√	√	√	√	√
WB-1 Solution (0.5 ml)	√	√	√	√	√	√	√	√
WB-2 Solution (50 ml)	√	√	√	√	√	√	√	√
5X Wash Solution (125 ml)	√	√	√	√	√	√	√	√
WestClear™ Nitrocellulose Membrane (0.2 μm, 7.5 x 8.5 cm, 5 sheets)			√	√	√	√	√	√
LumiSensor™ Chemiluminescent HRP Substrate (2 X 7.5 ml)			√	√
ChromoSensor™ One Solution TMB Substrate (15 ml)					√	√
SuperSensitive LumiSensor™ Chemiluminescent HRP Substrate ( 5 ml + 10 ml)							√	√
Dark Room & Film Requirement	**	**	√	√			√	√
User Manual	√	√	√	√	√	√	√	√

**Depending on the substrate you use, you need dark room and film if use chemiluminescent HRP substrate, but don't need if you use TMB substrate

Customer Testimonial

"GenScript Western kits have saved me weeks or months of research time! I absolutely love them! And the customer service is excellent, friendly and efficient."

― Dr. Sarah Yohannan, Yale School of Medicine.

ONE-HOUR Western™ Kit Accessories

GenScript provides a varity of reagents and related products for its line of ONE-HOUR Western™ Kits and ELISA Kits, including detection substrates, solutions and membranes.

Examples

Shown below is a western blot detection of housekeeping gene product GAPDH protein using LumiSensor™ Chemiluminescent HRP Substrate Kit.

Figure 1. 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and 0.08 μg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensor™ Chemiluminescent HRP Substrate Kit.
Western blot wash box and forceps.

Ordering Information

ONE-HOUR Western Detection Substrate

Cat. No.	Product Name	Price
L00221V60	LumiSensor^TM HRP Substrate Kit - 1 Kit (2 X 30ml)	$55.00
L00221V300	LumiSensor^TM HRP Substrate Kit - 1 Kit (2 X 150ml)	$145.00
L00221V500	LumiSensor^TM HRP Substrate Kit - 1 Kit (2 X 250ml)	$235.00
L00222V125	ChromoSensor^TM One-Solution TMB Substrate - 1 pk (125ml)	$39.00
L00222V250	ChromoSensor^TM One-Solution TMB Substrate - 1 pk (250ml)	$68.00
L00222V500	ChromoSensor^TM One-Solution TMB Substrate - 1 pk (500ml)	$119.00

Western Solutions

	Cat. No.	Product Name	Price
	MB01011	5X Wash Solution For One-Step Western Kits - 125 ml	$28.00
	M01013	Pretreat A(50 ml) & B(50 ml) Solution - 1 pk	$30.00

ONE-HOUR Western™ Kit Labware

Cat. No.	Product Name	Price
M00101	Western Wash Box (Blue) - 1 pack of 10	$80.00
M00102	Western Wash Box (Yellow) - 1 pack of 10	$80.00
M00099	Western Membrane Forceps - 1 pack of 10	$50.00
M00103	Western Wash Box (Black) - 1 pack of 10	$80.00

WestClear™ Nitrocellulose Membrane

	Cat. No.	Product Name	Price
	L00224A60	WestClear^TM Nitrocellulose Membrane - 1 pk (5 sheets)	$20.00

Technical Resources