** This is a software tool to create a scrambled sequence as negative control for your siRNA experiment.
Instruction:This program is designed to create a negative control for a siRNA. It accepts a short DNA sequence (<= 30 mer), and returns a scrambled sequence.
The scrambled sequence will have the following features:
- Having the same nucleotide composition as the input sequence.
- Passed the same siRNA filtering (e.g. no low complex sequence).
- Having the weakest (or no) match with any mRNA in the mRNA pool for a particular organism.
We will try up-to 1,000,000 combinations for the scrambling process to identify a really good candidate.