Off target prediction of current release of our design tool is only suitable to human, mouse and rat.
This option is valid if and only if the entrys human,mouse and rat in the above option are chosed. Entry "house" represents housekeeping genes which are expressed in all or near all tissues.
Previous investigations and GenScript experiment data show that siRNA "off target" transcript silencing mediated by seed region sequence is widespreed. "seed region" of siRNA is analogous to seed region of microRNA. GenScript resourchers found that size of seed region plays a major part in off target prediction. Size of seed region of siRNA we proposed is 6-8 bases.
Besides general sequence alignment, GenScript siRNA design tool incorporates a novel alignment approach, functional alignment. This idea for functional aligment derives from asymmetry of siRNA in the assembly of the RNAi enzyme complex.