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GANP® Transgenic Mice

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GenScript has licensed from Transgenic Inc. an unique GANP® technology for the production of high-affinity and high-specificity antibodies for our customers.

GANP, the acronym for Germinal Center Associated Nuclear Protein (GANP), is a recently discovered protein, over-expressed in B-cells centered in embryo. GANP carries a domain that generates RNA primers under the regulation of CD40/CD40L interaction (RNA-primase domain) and another domain of the MCM3-binding activity. It can be up-regulated in germinal center (GC) B cells upon immunization with T-cell dependent antigen. GANP can augment the induction of somatic hypermutation (SHM) in the V regions, resulting in the affinity maturation of V regions during the proliferation and differentiation of Ag-driven B cells in GCs. Accumulating research has suggested that GANP is important in the reconstruction of antibody genes, and transgenic mice expressing high level of GANP can be used to produce antibodies with high affinity and specificity.

GANP Mechanism

Key Features:

  • High affinity: GANP mouse produces mAbs with extraordinarily high affinity.
  • High specificity: GANP mouse antibodies can be used to establish high-specific ELISA system that distinguishes the similar substances.
  • High function activity: GANP mouse induces mAbs with better function activities, as proved by numerous case studies.

Case study:

  • GANP mouse produced extraordinarily higher affinity mAbs

    • GANP mouse
    (C57BL/6 strain)
    Wild-type mouse
    (C57BL/6 strain)
    BALB/c mouse
    Clone Association constant (KD) Clone Association constant (KD) Clone Association constant (KD)
    G1-22 1.77×10-6 W1-2 9.81×10-8 B1-10 5.68×10-9
    G1-68 4.81×10-7 W1-7 3.11×10-9 B2-24 1.64×10-7
    G1-124 1.01×10-6 W1-8 7.58×10-8 B2-27 4.97×10-6
    G1-165 1.14×10-7 W1-10 1.83×10-8    
    G1-181 1.09×10-8 W1-21 9.70×10-8    
    G1-231 2.07×10-6 W1-43 8.25×10-9    
    G2-10 9.90×10-11 W1-45 5.89×10-9    
    G2-25 1.51×10 -10 W1-63 5.67×10-9    
        W1-84 2.81×10-5    
    Table 1: Affinity test results for the mAb clones generated from GANP mouse, wild-type mouse and BALB/c mouse. GANP mouse produced extraordinarily higher affinity mAbs (Data from TransGenic Inc. )

  • GANP mouse produced highly specific antibody

    • GANP specificity
    Fig 1: The GANP mice antibodies have 5 times the specificity of those obtained from BALB/c mouse. Diacetlyspermine (blue) and two molecules (green and red) are extremely similar in both molecular weight and structure. (Data from TransGenic Inc. )

  • GANP mouse induces mAbs with higher neutralization activities.

    • GANP neutralization
    Fig 2: Neutralization activities of GANP mouse mAbs were clearly detected at 0.5mg/ml, more effective than those mAbs (W1-2 and W1-8) from C57BL/6. (Data from TransGenic Inc. )

    Guideline for GANP transgenic mouse offering

    GenScript has signed a license agreement with TransGenic Inc., and is authorized to use GANP® transgenic mice to provide custom antibody service worldwide (excluding Japan). According to the agreement, GenScript needs to report to Transgenic Inc. customer names who have used GANP service through GenScript. All clients can use GANP mice to generate high specificity and high affinity antibody more likely than using regular mice.

    Any customer who does contract research with GenScript using GANP mouse does not need to pay additional fee to Transgenic Inc. if the antibody is for research use only. However, Transgenic Inc. may charge additional license fees and/or royalties if the antibody becomes a commercial product. GenScript have no right to grant the commercial sublicense to any third part. The clients must negotiate with TransGenic Inc. to obtain their own commercial license. Currently GenScript is trying to get a commercial sublicense from TransGenic Inc. in order to mitigate customer's concern on future uncertainty.

    Quotation and Ordering:

      To request a quotation, please download and complete our Quick Quotation Request
      To order an mAb, please download & complete our Monoclonal Antibody Order Form
      To submit an antigen, mail it together with a completed hard copy of Antigen Submission
         Form to Antibody Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.

    Please email or fax all completed forms and quotation requests(including the Antigen Submission Forms) to GenScript.

      If you have any questions or would like to inquire about the status of your order, please contact us by phone, email, fax, or via our Secure Web Server.

    Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

    Email: antibody@genscript.com
    Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
    Fax: 1-732-210-0262 1-732-885-5878