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Gene-to-Protein

recombinant protein expression quotation

OptimumGeneTM Gene-to-Protein Service


Producing sufficient amount of proteins with desirable purity is often time-consuming and labor-intensive especially when a project starts from a piece of gene sequence. With proprietary OptimumGeneTM Codon Optimization and gene design technologies, GenScript's experienced team of scientist can help you speed up your project by offering guaranteed Gene-to-Protein service that delivers 10 mg of purified protein right out of your gene sequence.

GenScript's streamlined Gene-to-Protein service takes your project directly from gene synthesis to protein expression and purification and the whole project can be as low as $2,990. In addition, there are flexible scale-up options readily available to you. Simply submit your target gene sequence and 10 mg of purified protein will arrive at your bench in as little as 4 weeks.

To make our service more attractive to you, we recently increased the length of gene synthesis for OptimumGeneTM Gene-to-Protein Package from 400 bp to 1 kb without additional charge. Meanwhile, on top of the package's inherent 10 mg guarantee, you can also get an additional guarantee of 5 mg protein to meet your different needs if you choose to do so.

Advantages:


  • Gene-to-Protein Package
  • Comparison
  • OptimumGeneTM Technology
  • Customer Testimonial

OptimumGeneTM Gene-to-Protein Package (SC1484)

Milestones Timeline Specifications Deliverables

Step 1: Gene synthesis (FREE of charge up to 1 kb)

2–4 weeks1

  • Gene design and synthesis through the OptimumGeneTM Gene Design Technology
Gene in pUC57

Step 2: Subcloning

  • Subclone the gene into 3 expression vectors
Construct(s) report

Step 3: Expression optimization and expression verification

1–2 weeks
  • Transformation of 3 constructs into bacteria
  • Expression optimization: induction time, temperature, and IPTG concentration
  • Coomassie blue stained SDS-PAGE gel
  • Yield estimation and feasibility proposal for large-scale expression (optional)
QC data

Step 4: Expression and purification

1–2 weeks
  • Bacteria expression and one-step affinity purification
  • QC by SDS-PAGE, Western blot2, and MALDI-TOF3
5 mg/10 mg or more of purified soluble tagged protein4
QC data
Total price of OptimumGeneTM Gene-to-Protein Package: starting from $2,790 (5 mg)/$2,990 (10 mg)

1 Applicable to genes without complex sequences.

2 FREE Western QC data is available for tagged proteins that can be detected by the anti-His or anti-GST antibody. For other tagged proteins, additional charges may apply.

3 Optional MALDI-TOF data is available with additional charge.

4 Purification protocol provided by GenScript guarantees 5 mg/10 mg of tagged protein. If customers require small tags ( <20 aa) for purification, 1.5 mg/3 mg of protein is guaranteed. Extra fee is charged for additional amount of proteins.

Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.

OptimumGeneTM Gene-to-Protein Package vs Typical Services from Competitors (comparison based on a target gene of 1 kb in length):

OptimumGeneTM Gene-to-Protein
Package
Typical Services from Competitors
Gene Sequence Optimization
Yes
NA or with less effective technology
Timeline Reduction
1–2 weeks saved from shipping and streamlined services
10 mg Purified Protein Guarantee
Yes
No
One-stop Price
$2,990*
>20% higher

Comparison between DIY (do it yourself) and using GenScript OptimumGeneTM Gene-to-Protein Package (1 kb gene, using E. coli expression system):

DIY Genscript's Gene-to-Protein Package
Cost
Min. $5,000
Reagents cost (Min. $1,800) + Labor cost (Min. $3,200)**
$2,990*
Timeline
Lucky if complete in 8 weeks
As short as 4 weeks
Trouble Shooting
Frustrating
Free of Hassle
End Result
Not guaranteed
Guarantee 10 mg purified protein

* $2,990 includes <1 kb gene synthesis and 10 mg purified protein production, additional charges can apply for genes >1 kb in length. Please call for a custom quote.

** Reagent cost

  • $400 (traditional cloning for a 1 kb gene)
  • $600 (expression kit)
  • $450 (protein assay, including SDS-PAGE, WB, protein concentration detection, etc)
  • $150 (purification column)
  • $200 (PCR reagents, IPTG, culture medium, etc)

   Labor cost = $20/hr/FTE * 160 hrs

As one of the world's leading suppliers of gene synthesis services, GenScript has a proven record of dedication to innovation in our constant efforts to integrate new and updated technology into our gene expression repertoire. GenScript’s OptimumGeneTM Gene Design system is a proprietarily gene optimization technology that can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of productivity in any given expression system. The OptimumGeneTM algorithm takes into consideration of a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation.

codon optimization

Case Study:

Proven increase in protein expression through optimizing a variety of parameters

   codon optimization case study

codon optimization case study

Fig. 1. GenScript OptimumGene™ codon optimization genes increased the yield of expression (8 out of 10 genes) and the degree of solubility in some cases (6 out of 10 genes) compared to the native genes.

 

Reference: Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O. Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study. Protein Expr Purif. May 2008; 59(1): 94-102

View More Case Studies

Customer Testimonial


"GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service."

— Dr. Barry Bradford, Kansas State University Department of Animal Sciences & Industry


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Quotations and Ordering:

protein service quotation

protein services To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

protein services To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.

protein services If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.

protein services For questions or to inquire about the status of your order, contact us by email, phone, fax or via our secure online messaging system.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Order by mail Email: protein@genscript.com
Order by phone Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Order by fax Fax: 1-732-210-0262 1-732-885-5878