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OptimumGene^TM Gene-to-Protein Service

Producing sufficient amount of proteins with desirable purity is often time-consuming and labor-intensive especially when a project starts from a piece of gene sequence. With proprietary OptimumGene^TM Codon Optimization and gene design technologies, GenScript's experienced team of scientist can help you speed up your project by offering guaranteed Gene-to-Protein service that delivers 10 mg of purified protein right out of your gene sequence.

GenScript's streamlined Gene-to-Protein service takes your project directly from gene synthesis to protein expression and purification and the whole project can be as low as $2,990. In addition, there are flexible scale-up options readily available to you. Simply submit your target gene sequence and 10 mg of purified protein will arrive at your bench in as little as 4 weeks.

To make our service more attractive to you, we recently increased the length of gene synthesis for OptimumGene^TM Gene-to-Protein Package from 400 bp to 1 kb without additional charge. Meanwhile, on top of the package's inherent 10 mg guarantee, you can also get an additional guarantee of 5 mg protein to meet your different needs if you choose to do so.

Advantages:

Proprietary OptimumGene^TM Codon Optimization Technology
Guaranteed 10 mg of purified proteins, starting from $2,990
Fast turnaround: as little as 4 weeks
One-stop service platform: GenScript can take your project directly from gene synthesis to downstream activities including recombinant protein expression, stable cell line generation, antibody production, and assay development.

Gene-to-Protein Package
Comparison
OptimumGene^TM Technology
Customer Testimonial

OptimumGene^TM Gene-to-Protein Package (SC1484)

Milestones	Timeline	Specifications	Deliverables
Step 1: Gene synthesis (FREE of charge up to 1 kb)	2–4 weeks¹	Gene design and synthesis through the OptimumGene^TM Gene Design Technology	Gene in pUC57
Step 2: Subcloning	2–4 weeks¹	Subclone the gene into 3 expression vectors	Construct(s) report
Step 3: Expression optimization and expression verification	1–2 weeks	Transformation of 3 constructs into bacteria Expression optimization: induction time, temperature, and IPTG concentration Coomassie blue stained SDS-PAGE gel Yield estimation and feasibility proposal for large-scale expression (optional)	QC data
Step 4: Expression and purification	1–2 weeks	Bacteria expression and one-step affinity purification QC by SDS-PAGE, Western blot², and MALDI-TOF³	5 mg/10 mg or more of purified soluble tagged protein⁴ QC data
Total price of OptimumGene^TM Gene-to-Protein Package: starting from $2,790 (5 mg)/$2,990 (10 mg)
¹ Applicable to genes without complex sequences. ² FREE Western QC data is available for tagged proteins that can be detected by the anti-His or anti-GST antibody. For other tagged proteins, additional charges may apply. ³ Optional MALDI-TOF data is available with additional charge. ⁴ Purification protocol provided by GenScript guarantees 5 mg/10 mg of tagged protein. If customers require small tags ( <20 aa) for purification, 1.5 mg/3 mg of protein is guaranteed. Extra fee is charged for additional amount of proteins.

Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.

OptimumGene^TM Gene-to-Protein Package vs Typical Services from Competitors (comparison based on a target gene of 1 kb in length):

	OptimumGene^TM Gene-to-Protein Package	Typical Services from Competitors
Gene Sequence Optimization	Yes	NA or with less effective technology
Timeline Reduction	1–2 weeks saved from shipping and streamlined services	—
10 mg Purified Protein Guarantee	Yes	No
One-stop Price	$2,990*	>20% higher

Comparison between DIY (do it yourself) and using GenScript OptimumGene^TM Gene-to-Protein Package (1 kb gene, using E. coli expression system):

	DIY	Genscript's Gene-to-Protein Package
Cost	Min. $5,000 Reagents cost (Min. $1,800) + Labor cost (Min. $3,200)**	$2,990*
Timeline	Lucky if complete in 8 weeks	As short as 4 weeks
Trouble Shooting	Frustrating	Free of Hassle
End Result	Not guaranteed	Guarantee 10 mg purified protein

* $2,990 includes <1 kb gene synthesis and 10 mg purified protein production, additional charges can apply for genes >1 kb in length. Please call for a custom quote.

** Reagent cost

$400 (traditional cloning for a 1 kb gene)
$600 (expression kit)
$450 (protein assay, including SDS-PAGE, WB, protein concentration detection, etc)
$150 (purification column)
$200 (PCR reagents, IPTG, culture medium, etc)

Labor cost = $20/hr/FTE * 160 hrs

As one of the world's leading suppliers of gene synthesis services, GenScript has a proven record of dedication to innovation in our constant efforts to integrate new and updated technology into our gene expression repertoire. GenScript’s OptimumGene^TM Gene Design system is a proprietarily gene optimization technology that can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of productivity in any given expression system. The OptimumGene^TM algorithm takes into consideration of a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation.

Case Study:

Proven increase in protein expression through optimizing a variety of parameters

Fig. 1. GenScript OptimumGene™ codon optimization genes increased the yield of expression (8 out of 10 genes) and the degree of solubility in some cases (6 out of 10 genes) compared to the native genes.

Reference: Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O. Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study. Protein Expr Purif. May 2008; 59(1): 94-102

View More Case Studies

Customer Testimonial

"GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service."

— Dr. Barry Bradford, Kansas State University Department of Animal Sciences & Industry

Partial publications cited our protein services

Flavia Fontanesi, Iliana C. Soto, Darryl Horn, and Antoni Barrientos. Mss51 and Ssc1 facilitate translational regulation of cytochrome c oxidase biogenesis. Mol. Cell. Biol. Jan 2010; 30(1): 245 - 259

Yanchun Li, Darren F. Boehning, Ting Qian, Vsevolod L. Popov, and Steven A. Weinman. Hepatitis C virus core protein increases mitochondrial ROS production by stimulation of Ca2+ uniporter activity FASEB. J. Aug 2007: 2474 - 2485

More publications

Quotations and Ordering:

To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.

If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.

For questions or to inquire about the status of your order, contact us by email, phone, fax or via our secure online messaging system.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

	Email: protein@genscript.com
	Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
	Fax: 1-732-210-0262 1-732-885-5878

OptimumGeneTM Gene-to-Protein Service