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cMyc tag antibody

THE™ cMyc Tag Antibody (Mouse)

cMyc tag antibody

The best quality in the industry, THE™ cMyc tag antibody is one member of GenScript's THE™ Elite antibodies. The immunogen of THE™ cMyc tag antibody is a synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH. THE™ cMyc tag antibody is suitable for detecting the expression level of c-Myc or its fusion proteins where the c-Myc tag is terminal or internal. This antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.

Key Features:

  • High Specificity: No non-specific binding
  • High Effect: 1:20 000 diluted from 1.0 mg/ml can get good signal in WB analysis
  • High Sensitivity: Detection level as low as 5 ng
  • Broad Reactivity: Recognization of N-terminal, internal and C-teminal c-Myc tagged protein
  • Long-term Stability: Stability of lyophilized form for at least two years
  • Rich Applications: ELISA, Western blotting, Immunoprecipitation, Immunofluorescence, etc.

  • Product Details
  • Examples

Ordering Information:

All 40μg antibodies are under 40% off promotion!

Cat. No.
Product Name
Size
Price
Quantity
A00704-100 THETM c-Myc Tag Antibody, mAb, Mouse
100 μg
$175.00
A00863-100 THETM c-Myc Antibody [HRP], mAb, Mouse
100 μg
$410.00
A00864-40 THETM c-Myc Antibody [Biotin], mAb, Mouse
40 μg
$74.00 $44.4
A00864-200 THETM c-Myc Antibody [Biotin], mAb, Mouse
200 μg
$333.00


Related Information:

Cat. No.
Product Name
Size
Price
Quantity
A00172-40 c-Myc-tag Antibody, pAb, Rabbit
40 μg
$79.00 $47.4
A00172-100 c-Myc-tag Antibody, pAb, Rabbit
100 μg
$198.00
A00172-200 c-Myc-tag Antibody, pAb, Rabbit
200 μg (5x40μg)
$275.00
A00173-100 c-Myc-tag Antibody [HRP], pAb, Rabbit
100 μg
$218.00

  Examples:

  1. Sensitivity Detection


cMyc tag antibody

cMyc tag antibody
 

Sensitivity analysis of THE™ c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.

A: 0.2 μg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 μg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 μg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)

The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG

 

  2. Reactivity Test

 
cMyc tag antibody
 

Western blot discovers working concentration comparison of THE™ c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.

Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 μg/ml) in assay.

The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

 

  3. Immunoprecipitation Application

cMyc tag antibody

Lane 1-4: 0.5, 0.2, 0.1, and 0.05 μg Multiple Tag Cell Lysate (GenScript, M0100) as western blot control
Lane 5-7: 1, 0.5, and 0.25 μg

THE™ cMyc tag antibody (Mouse) (GenScript, A00704) with 20 μg Multiple Tag Cell Lysate (GenScript, M0100) for immunoprecipitation analysis performed by One-Step IP-Western Kit (Mouse)(GenScript, L00232)

 

  4. Immunofluorescence Staining

cMyc tag antibody

Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using THE™ cMyc tag antibody (Cat. No. A00704).