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GAPDH Antibody [HRP], pAb, Goat

*This product has been discontinued!*
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
A00192
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Overview
Specificity GenScript Goat Anti-GAPDH [HRP] Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.
Host Species Goat
Immunogen Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle
Species Reactivity Human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli
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Properties
Concentration 0.5 mg/ml, lyophilized with PBS, pH7.4, containing 1%BSA, 0.01% thimerosal
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
Purification Immunoaffinity chromatography
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Background
Target Background Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Synonyms Anti-GAPDH [HRP];
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Citations
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ChengJie, et al. Tryptophan derivatives regulate the transcription of Oct4 in stem-like cancer cells. Nat Commun. (2015-06)
ChoiHyong Woo, et al. Human GAPDH Is a Target of Aspirin's Primary Metabolite Salicylic Acid and Its Derivatives. PLoS ONE. (2015)
Reusch JA, et al. Cellular Differentiation Regulator BLIMP1 Induces Epstein-Barr Virus Lytic Reactivation in Epithelial and B Cells by Activating Transcription from both the R and Z Promoters. J Virol. (2014-12)
Ng MH, et al. Activation of Ras-dependent Elk-1 activity by MLL-AF4 family fusion oncoproteins. Exp Hematol. (2010-06)
Chen YC, et al. OCT4 expression maintained cancer stem-like properties in hepatoma cell line. PLoS One. (2008-07)
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