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GST-tag Antibody [Biotin], pAb, Rabbit

*This product has been discontinued!*
This antibody is specific for the GST protein from Schistosoma japonicum and is useful for detecting GST and GST-fusion proteins.Cross-reactivity with cell lysate of E.coli bacteria, sf21 cell and yeast have not been observed.
A00202
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Overview
Specificity This antibody is specific for the GST protein from Schistosoma japonicum and is useful for detecting GST and GST-fusion proteins.Cross-reactivity with cell lysate of E.coli bacteria, sf21 cell and yeast have not been observed.
Host Species Rabbit
Immunogen Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum
Species Reactivity Schistosoma japonicum
Conjugate Biotin
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Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.1 µg/ml
Western Blot 0.1-1.0 µg/ml
Other applications User-optimized
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Properties
Form Liquid
Storage Buffer 1×PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Concentration 1 mg/ml
Storage Instructions The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap.
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Background
Target Background Rabbit Anti-GST-tag [Biotin] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to biotin.
Synonyms Anti-GST-tag [Biotin];
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Yang Luo, et al. Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor. Cell Chem Biol. (2020-12)
Machida K., et al. In-Solution SH2 Domain Binding Assay Based on Proximity Ligation. Methods Mol Biol. (2017)
Thompson CM, et al. SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR. BMC Biotechnology. (2015-06)