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A00619
200 μg
$69
HRP Antibody (Horseradish Peroxidase), pAb, Rabbitcustom antibody production
HRP Antibody (Horseradish Peroxidase), pAb, Rabbit
Full Name HRP Antibody (Horseradish Peroxidase), pAb, Rabbit
Abbreviated name-1
Rabbit Anti-HRP;
Documents
HRP Antibody (Horseradish Peroxidase), pAb, RabbitDocument-MSDS: 8002_20081105023644.PDF (PDF)
HRP Antibody (Horseradish Peroxidase), pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
HRP Antibody (Horseradish Peroxidase), pAb, Rabbit zoomHRP Antibody (Horseradish Peroxidase), pAb, Rabbit
Reference
Description
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator enzyme in reactions in which peroxide is produced, such as in conjunction with glucose oxidase in the evaluation of glucose in biological fluids. It also can be used as an enzyme label, such as in ELISA systems and peroxidase-anti-peroxidase complexes in immunohistochemistry.
GenScript Rabbit Anti-HRP Polyclonal Antibody is developed in rabbit hosts using purified peroxidase from horseradish as the immunogen.
GenScript Rabbit Anti-HRP Polyclonal Antibody is purified from rabbit antiserum by Protein G chromatography and is supplied as a 200 μg aliquot at a concentration of 0.5 mg/ml.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-HRP Polyclonal Antibody is specific to peroxidase (horseradish).
Concentration
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
Immunogen
Purified peroxidase from horseradish
Purification Method
Protein G chromatography
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Other applications: user-optimized
Conjugation
Unconjugated
Host Species
Rabbit
Note
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
1
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2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
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