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HRP Antibody (Horseradish Peroxidase), pAb, Rabbit  |
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| Full Name |
HRP Antibody (Horseradish Peroxidase), pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-HRP; |
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Description |
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator enzyme in reactions in which peroxide is produced, such as in conjunction with glucose oxidase in the evaluation of glucose in biological fluids. It also can be used as an enzyme label, such as in ELISA systems and peroxidase-anti-peroxidase complexes in immunohistochemistry.
GenScript Rabbit Anti-HRP Polyclonal Antibody is developed in rabbit hosts using purified peroxidase from horseradish as the immunogen.
GenScript Rabbit Anti-HRP Polyclonal Antibody is purified from rabbit antiserum by Protein G chromatography and is supplied as a 200 μg aliquot at a concentration of 0.5 mg/ml. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-HRP Polyclonal Antibody is specific to peroxidase (horseradish). |
Concentration |
0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide |
Immunogen |
Purified peroxidase from horseradish |
Purification Method |
Protein G chromatography |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8002_20081105023644.PDF (PDF)