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SUMO-tag Antibody, pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti-SUMO-tag pAb;

Documents
Document-MSDS: 4133_20080228013647.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

Small ubiquitin-related modifier (SUMO) proteins are conjugated to numerous intracellular targets and serve to modulate protein interaction, localization, activity, and stability. SUMO (also known as 'Smt3' and 'sentrin' in other organisms) is linked to several different pathways, including nucleocytoplasmic transport. The attachment of SUMO to targets proteins is stimulated by (protein inhibitor of activated STATs PIAS) proteins that serve as E3-like ligases.
GenScript Rabbit Anti-SUMO-tag Polyclonal Antibody is developed in rabbit s using purified recombinant yeast SUMO protein.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

Rabbit Anti-SUMO-tag Polyclonal Antibody specifically reacts with fusion proteins containing SUMO protein.

Concentration

5 mg/ml in PBS, pH7.4, containing 0.02% sodium azide

Immunogen

Purified recombinant yeast SUMO

Purification Method

Protein G chromatography

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:5,000-1:20,000
Western blot: 1:1,000-1:10,000
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µl of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Conjugation

Unconjugated

Host Species

Rabbit

Antigen Species

Yeast

Note

Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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