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PKC ε Antibody, pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti-PKC ε pAb; Anti-PKC ε polyclonal antibody

Documents
Document-MSDS: 4227_20080228213118.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference
Breitkreutz D. et al., 2007, J Cancer Res Clin Oncol. 133:793-808 Basu A. and Sivaprasad U., 2007, Cell Sinal. 19:1633-1642

Description

Protein kinase C (PKC), a family of serine/threonine kinases, plays an important role in the growth factor signal transduction pathway. The PKC family consists of at least ten members, divided into three subgroups: classical PKCs (alpha, beta I, beta II, gamma), novel PKCs (delta, epsilon, eta, theta), and atypical PKCs (zeta, iota/lambda). Accumulating data suggest that various PKC isoforms participate in the regulation of cell proliferation, differentiation, survival and death. PKC epsilon (PKCε) is a calcium-independent and phorbolester/diacylglycerol-sensitive serine/threonine kinase. PKCε is the only PKC isozyme that has been shown to behave as an oncoprotein. The Rabbit anti-PKC ε antibody is developed in rabbit using a synthetic peptide, KGFSYFGEDLMP, corresponding to a distinct C terminal region from rat PKC ε conjugated to KLH. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-PKC ε reacts with mouse and rat PKC ε. Sequence homology predicts that it will also react with human and rabbit PKC ε.

Concentration

1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

A synthetic peptide, KGFSYFGEDLMP, corresponding to amino acids 726 - 737 of rat PKC ε conjugated to KLH

Purification Method

Immunoaffinity chromatography

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Conjugation

Unconjugated

Host Species

Rabbit

Antigen Species

Human

Note

Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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