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tag-100-tag Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A00677-200	tag-100-tag Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A00677-40	tag-100-tag Antibody, pAb, Rabbit	40 μg	$50

Full Name

tag-100-tag Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-tag-100-tag pAb;

Documents
Document-MSDS: 8068_20081107010654.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems. Tag-100 tag is an epitope tag composed of a 12-residue peptide, EETARFQPGYRS, derived from the C-termini of mammalian MAPK/ERK kinases.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

This Antibody recognizes Tag-100 tagged fusion proteins.

Concentration

1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide

Immunogen

Tag-100-tag peptide EETARFQPGYRS conjugated to KLH

Purification Method

Immunoaffinity chromatography

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR Western^TM Kit: For quick results, ONE-HOUR Western^TM Complete Kit (GenScript, L00204C) is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized

Conjugation

Unconjugated

Host Species

Rabbit

Antigen Species

Human

Note

Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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