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Name
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A00866-100
100 µg
$410
THETM GST Antibody [HRP], mAb, Mousecustom antibody production
 THE<sup>TM</sup> GST Antibody [HRP], mAb, Mouse
Full Name THETM GST Antibody [HRP], mAb, Mouse
Abbreviated name-1
Mouse Anti-GST [HRP] mAb;
Documents
 THE<sup>TM</sup> GST Antibody [HRP], mAb, MouseDocument-MSDS: 8293_20081117222014.PDF (PDF)
 THE<sup>TM</sup> GST Antibody [HRP], mAb, MouseDocument-MSDS: 9195_20090114012119.PDF (PDF)
 THE<sup>TM</sup> GST Antibody [HRP], mAb, MouseDocument-COA: 11K000424_A00866_COA.pdf (pdf)
Figures
 THE<sup>TM</sup> GST Antibody [HRP], mAb, Mouse zoom THE<sup>TM</sup> GST Antibody [HRP], mAb, Mouse
Reference
Description
GenScript THETM GST Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of GST in fusion proteins.
Ig Subclass
IgG1
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
THETM GST Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Concentration
0.5mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Immunogen
Recombinant GST protein (26 kD)
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Conjugation
Horseradish peroxidase (HRP)
Host Species
Mouse
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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