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E-tag Antibody [HRP], pAb, Rabbit

Cat. No.	Name	Size	Price
A00874-200	E-tag Antibody [HRP], pAb, Rabbit	200 μg(5x40μg)	$248
A00874-40	E-tag Antibody [HRP], pAb, Rabbit	40 μg	$55

Full Name

E-tag Antibody [HRP], pAb, Rabbit

Abbreviated name-1

Rabbit Anti-E-tag [HRP] pAb;

Documents
Document-MSDS: 8299_20081118022322.PDF (PDF)
Document-MSDS: 9195_20090114012119.PDF (PDF)
Document-COA: 15694_20120314050900.PDF (PDF)
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Reference

Description

GenScript Rabbit Anti-E-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-E-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E-tags.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Anti-E-tag [HRP] Polyclonal Antibody recognizes E tagged fusion proteins.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Immunogen

E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Conjugation

Horseradish peroxidase (HRP)

Host Species

Rabbit

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.