You are here: Products » Antibodies » Epitope Tag Antibodies » Rabbit Anti-Strep II pAb [HRP]

NWSHPQFEK Antibody [HRP], pAb, Rabbit

Cat. No.	Name	Size	Price
A00875-200	NWSHPQFEK Antibody [HRP], pAb, Rabbit	200 μg(5x40μg)	$248
A00875-40	NWSHPQFEK Antibody [HRP], pAb, Rabbit	40 μg	$55

Full Name

NWSHPQFEK Antibody [HRP], pAb, Rabbit

Abbreviated name-1

Rabbit anti NWSHPQFEK [HRP] pAb;

Documents
Document-MSDS: 9904_20090531225840.PDF (PDF)
Figures
zoom
Reference

Description

Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. The Rabbit anti NWSHPQFEK polyclonal antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain NWSHPQFEK.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

This Antibody recognizes Strep-tag II tagged fusion proteins.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Immunogen

Strep-tag II epitope peptide NWSHPQFEK conjugated to KLH.

Purification Method

Immunoaffinity

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1.0 µg/ml
Other Applications: user-optimized

Conjugation

Horseradish peroxidase (HRP)

Host Species

Rabbit

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

Related Products

Cat. No.	Name	Size	Price
A00626-40	NWSHPQFEK Antibody, pAb, Rabbit	40 μg	$50
A00626-200	NWSHPQFEK Antibody, pAb, Rabbit	200 μg(5x40μg)	$225


1	Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.